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Research Articles

Urine Cotinine Underestimates Exposure to the Tobacco-Derived Lung Carcinogen 4-(Methylnitrosamino)-1-(3-Pyridyl)-1-Butanone in Passive Compared with Active Smokers

Neal Benowitz, Maciej Lukasz Goniewicz, Mark D. Eisner, Eduardo Lazcano-Ponce, Wioleta Zielinska-Danch, Bartosz Koszowski, Andrzej Sobczak, Christopher Havel and Peyton Jacob III
Neal Benowitz
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Maciej Lukasz Goniewicz
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Mark D. Eisner
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Eduardo Lazcano-Ponce
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Wioleta Zielinska-Danch
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Bartosz Koszowski
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Andrzej Sobczak
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Christopher Havel
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Peyton Jacob
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DOI: 10.1158/1055-9965.EPI-10-0497 Published November 2010
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Abstract

Objectives: Cotinine and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) are widely used biomarkers for tobacco-derived nicotine and the lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), respectively. The discrepancy between cotinine levels in relation to disease risk comparing active versus passive smoking suggests a nonlinear tobacco smoke dose-response and/or that cotinine is not providing an accurate measure of exposure to the toxic constituents of secondhand tobacco smoke.

Methods: Cotinine and NNAL were measured in the urine of 373 active smokers and 228 passive smokers.

Results: Average cotinine levels were 1,155 (interquartile range, 703-2,715) for active smokers and 1.82 (0.45-7.33) ng/mg creatinine for passive smokers. Average NNAL levels were 183 (103-393) and 5.19 (2.04-11.6) pg/mg creatinine, respectively. NNAL/cotinine ratio in urine was significantly higher for passive smokers when compared with active smokers (2.85 × 103 versus 0.16 × 103, P < 0.0001).

Conclusions: Passive smoking is associated with a much higher ratio of NNAL/cotinine in the urine compared with active smoking.

Impact: Cotinine measurement leads to an underestimation of exposure to the carcinogen NNK from secondhand smoke when compared with active smoking. Cancer Epidemiol Biomarkers Prev; 19(11); 2795–800. ©2010 AACR.

Introduction

Cotinine, the major proximate metabolite of nicotine, is widely used as a biomarker of tobacco exposure (1, 2). Measurement of cotinine in blood, saliva, or urine, has been used to support epidemiologic findings of causal relationships between secondhand smoke (SHS) exposure and lung cancer, cardiovascular disease, and aggravation of chronic obstructive pulmonary disease (COPD) in adults, and asthma in children (3-6).

If one measures the concentrations of cotinine in people exposed to SHS compared with active smoking, the relative exposure with SHS exposure is typically 1% or less, comparing blood or urine cotinine levels in SHS versus active smokers (1). In contrast, the relative risk of lung cancer and cardiovascular disease with SHS exposure compared with active smoking is much higher than the relative cotinine levels. For example, the odds ratio for cigarette smoking and lung cancer is 1.25 for SHS exposure and 10 for active smoking, translating into a relative attributable risk ratio of 36 (7). The odds ratio for cardiovascular disease is about 1.3 with SHS and 2 with active smoking, translating into a relative attributable risk ratio of 3.3 (8). The discrepancy between relative cotinine levels and disease risk with SHS versus active smoking suggests that there is a nonlinear tobacco smoke dose-response and/or that cotinine is not providing an accurate measure of exposure to the toxic constituents of tobacco smoke.

Among the most important tobacco smoke toxins are tobacco-specific nitrosamines, which are derived from nicotine and other tobacco alkaloids mostly during tobacco curing (9). These compounds are known animal and human carcinogens. The most potent carcinogenic tobacco-specific nitrosamine is 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). NNK is metabolized in the human body to 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), which is further conjugated to form NNAL N- and O-glucuronides (NNAL-Gluc). The presence of NNAL in urine is highly specific for tobacco exposure and provides a biochemical link between both active and passive smoking and lung cancer (3).

There are several reasons to suspect that cotinine levels underestimate exposure to NNK. As SHS ages, concentrations of nicotine decline faster than many other gaseous and particulate components of smoke due to the absorption of nicotine on surfaces such as walls and carpets (10). In addition, NNK levels increase as SHS ages, presumably related to the reaction of nicotine with nitric oxide in SHS (11, 12). Thus, exposure to SHS over a period of time, in between emissions from individual cigarettes, would be expected to result in lower relative intake of nicotine versus NNK as compared with that obtained by the active smoker from mainstream smoke. Additionally, the half-life of NNAL is much longer (10 d) than that of cotinine (16 h; refs. 2, 13). As a result, with intermittent exposure to SHS, cotinine levels will decline between exposures to a much greater extent than NNAL, which would further increase the ratio of NNAL/cotinine in passive versus active smokers.

The aim of our study was to examine the hypothesis that cotinine does not provide an accurate assessment of exposure to the tobacco smoke carcinogen NNK in passive smokers. We compared the relative concentrations of NNAL and cotinine in active versus passive smokers.

Materials and Methods

Study design and subjects

A descriptive study was conducted in smoking and nonsmoking subjects from the United States, Poland, and Mexico, each of whom provided a urine sample for the measurements of concentration of NNAL and cotinine. The subjects included 373 smokers and 228 nonsmokers with evidence of exposure to SHS. Detailed characteristics of the study groups are presented in Table 1.

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Table 1.

Characteristics of the study groups (n = 601)

Adult daily smokers were recruited in three different studies (studies A-C). The groups were selected to include both regular daily smokers and occasional smokers. Study A was a study of tobacco smoke biomarkers comparing African American and white smokers in San Francisco (n = 128). Study B assessed the effect of smoking topography on tobacco biomarkers among daily smokers recruited in the Silesia region, Poland (n = 187, Koszowski and Sobczak). Study C compared urine biomarkers in daily compared with occasional smokers in Pittsburgh (n = 58, Shiffman).

Nonsmokers exposed to SHS had participated in two previously published (studies D and F) and one unpublished (study E) studies in which urine was collected for the assessment of SHS exposure. Subjects were selected to range from very light to heavy SHS exposure. Study D was a U.S. cohort study of nonsmoking adults with COPD, who collected samples in their homes and mailed samples to the investigators (n = 72; ref. 14). Among recruited subjects, there were no current smokers, although almost half of them had a history of smoking (n = 35; refs. 1, 2). Only subjects who had biochemical evidence of SHS exposure (cotinine or NNAL levels above the limit of quantitation) were included. Study group E was a Polish cohort of nonsmoking adults with urine samples to assess secondhand exposure to tobacco smoke in home and work environments. Subjects provided morning spot samples during screening medical tests (n = 108, Zielinska-Danch). Study F included volunteers from central Mexico who provided a urine sample after being in a discotheque in which smoking was ongoing for at least 1 hour (n = 81; ref. 15).

Analytical chemistry

Cotinine (unconjugated) was analyzed by liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry by a method similar to the one described by Bernert et al. (16). Total NNAL (after hydrolysis of NNAL glucuronide) was analyzed by liquid chromatography-tandem mass spectrometry as described by Jacob et al. (17). The limits of quantification were 0.05 ng/mL and 0.25 pg/mL for cotinine and NNAL, respectively.

Data analysis

Nonparametric Kruskal-Wallis ANOVA was used to compare biomarker levels and their ratios among study groups. Pearson correlations of log-transformed urine cotinine and NNAL concentrations were done. All analyses were done using STATISTICA data analysis software system v. 8 (StatSoft, Inc.).

Results

Geometric mean urine cotinine and NNAL concentrations and the ratio of NNAL/cotinine for each of the study groups are shown in Table 1, and comparisons between active and passive smokers in Fig. 1. The distribution of the NNAL/cotinine ratio among all subjects is shown in Fig. 2. The average ratio of NNAL/cotinine was almost 18 times higher in passive smokers (2.85; interquartile range, 1.32-6.14 × 103) compared with active smokers (0.16; interquartile range, 0.09-0.26 × 103; P < 0.0001), and there was relatively little overlap in the ratio in the active versus passive smoking groups. The two subjects with the highest NNAL/cotinine ratios (361 and 107 × 103) were from passive smoker groups D and E, respectively; and their high ratios were due to extraordinarily high levels of urine NNAL.

Figure 1.
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Figure 1.

Comparison of geometric means of cotinine, NNAL and NNAL/cotinine ratios among self-reported active (n = 373) and passive smokers (n = 228; bars show interquartile ranges).

Figure 2.
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Figure 2.

Distribution of urine NNAL/cotinine ratio among self-reported active (n = 373) and passive smokers (n = 228).

Among active smokers, urine cotinine/creatinine ratios were significantly higher for group A compared with groups B and C (P < 0.05) and urine NNAL/creatinine ratios were significantly higher in group C compared with groups A and B (P < 0.05). Among smokers, the urine NNAL/cotinine ratio was significantly higher in groups B and C compared with group A (P < 0.05). Within group A, there was no significant difference in the NNAL/cotinine ratio comparing African American and white smokers. Among passive smokers, group F had significantly higher urine cotinine and NNAL levels and lower NNAL/cotinine ratios compared with groups D and E (all P < 0.05).

Among active smokers, log-transformed concentrations of cotinine and NNAL urine were highly correlated: group A, r = 0.79; group B, r = 0.80; group C, r = 0.79; all smokers, r = 0.76 (all P < 0.05). Among passive smokers, these biomarkers were also highly correlated: group D, r = 0.71; group E, r = 0.74; group F, r = 0.67; all passive smokers, r = 0.79 (all P < 0.05).

Discussion

The main and novel finding of our study is that the ratio of NNAL to cotinine is much higher in passive compared with active cigarette smokers. This finding supports the idea that passive smoking leads to relatively higher exposure to the carcinogen NNK in relation to nicotine compared with active smoking. Thus, the use of urine cotinine to estimate exposure to the tobacco smoke carcinogen NNK in passive smokers with the assumption of a similar relationship between nicotine and NNK as that seen in active smokers, leads to an underestimation of exposure and potential cancer risk. Our data on absolute concentrations of NNAL and cotinine in urine among active and passive smokers are similar to those reported by other researchers (1, 18); however, to the best of our knowledge, no one has published a formal comparison of ratios in active and passive smokers.

Our study focuses on NNAL, which is a highly specific biomarker of tobacco smoke exposure. We do not know if cotinine measurement also underestimates exposure to other tobacco smoke toxins based on biomarker measures in humans because the other toxins are not specific to tobacco exposure. However, studies of the concentrations of nicotine and other tobacco smoke constituents in the air immediately after smoking and then followed over time indicate that nicotine levels decline much faster than do most other smoke constituents (10). Thus, it is likely that measurement of cotinine, which reflects intake of nicotine, also underestimates exposure to other tobacco smoke toxins. Epidemiologic data comparing risks of lung cancer and cardiovascular disease in SHS exposed versus active smokers show much higher risk for SHS compared with active smokers based on relative cotinine levels (7, 8). Thus, cotinine underestimates the risk of tobacco-related diseases in passive smokers compared with active smokers.

On average for U.S. cigarettes, the mainstream/sidestream smoke ratios for nicotine and NNK are 2.31 and 0.40, respectively (19). Given these ratios and based on exposure to fresh sidestream smoke, one would expect that passive smokers would be exposed to relatively more nicotine than NNK compared with active smokers, which is opposite to what we observed. As described above, as SHS ages nicotine levels decline while NNK levels increase, which most likely explains our observations.

Our study has potential limitations with respect to generalizability. Our subjects were not representative of the general U.S. population. Our active smokers came from both the United States and Poland. Our SHS-exposed subjects were also multinational, with different levels of SHS exposure. These included heavily exposed (Mexican discotheques), moderately exposed (Polish workers), and lightly exposed (COPD subjects in the United States). The duration of exposure to SHS was also different in these three groups. The Mexican subjects were exposed for a short period of time, the Polish and COPD subjects were presumably exposed over a longer period of time. Furthermore, it is unknown if this is an effect of COPD on the absorption of nicotine or NNK.

Comparison of the urine NNAL/cotinine ratio within the three groups of smokers indicated that Polish regular smokers had a significantly higher ratio than U.S. regular smokers. These groups had similar NNAL levels but the U.S. smokers had, on average, higher cotinine levels. This difference between groups of smokers could be due to the difference in racial composition [the U.S. group included nearly half African Americans, who metabolize cotinine and possibly NNAL more slowly than whites (refs. 20, 21), and/or due to differences in NNK levels in American versus Polish cigarettes]. However, we found no difference in the NNAL/cotinine ratio comparing African American to white smokers, suggesting that racial differences in metabolism do not explain our findings. The NNK content of cigarette tobacco and the NNK yield of cigarettes by machine-testing are known to differ in different countries (22, 23). However, we were unable to locate any published data on NNK content of Polish cigarettes. Of note is that our occasional U.S. smokers had a NNAL/cotinine ratio similar to Polish regular smokers.

Among the passive smokers, the two groups of lightly exposed smokers in the United States and Poland had similar ratios, but the heavily exposed Mexican group had a significantly higher ratio than either of the other two groups. Cigarettes from Mexico are reported to have lower NNK content and yield than U.S. cigarettes (22, 23). Despite lower NNK levels in the tobacco, the NNAL/cotinine ratios were higher in Mexican passive smokers, indicating that country differences cannot explain our results. Considering all of our groups of smokers and nonsmokers, there seems to be a pattern going from passive smokers to active smokers, such that the heavier the exposure, the lower the NNAL/cotinine ratio.

In our interpretation of the study findings, we make the assumption that urine NNAL levels quantitatively reflect systemic NNK exposure, which is expected because NNAL is a metabolite of NNAL. In active smokers, urine NNAL is strongly correlated with mouth-level exposure to NNK, as assessed by measuring the latter in cigarette butts (24). The possibility of dose-dependent metabolism of NNK such that there is a saturation of metabolism at high doses with relatively less excretion of NNAL might be considered as a potential confounder. We are aware of only one study that has examined dose-dependent metabolism of NNK, a study carried out in rodents (25). This study found that urine recovery of NNAL was greater with higher compared with lower doses of NNK. If this is also the case for people, it would bias against our findings of higher NNAL/cotinine ratios in those exposed to lower compared with higher levels of tobacco smoke. Thus, the dose-dependent metabolism of NNK does not seem to explain our findings.

In conclusion, passive smoking is associated with a much higher ratio of NNAL/cotinine in the urine compared with active smoking. This finding is robust and is seen even with the inclusion of subjects from different countries and with different levels of active and passive smoke exposures. Even the most heavily exposed passive smokers had much lower ratios than occasional light active smokers, supporting the idea that the use of cotinine measurement leads to an underestimation of exposure to the carcinogen NNK from SHS when compared with active smoking.

Disclosure of Potential Conflicts of Interest

N.L. Benowitz, tobacco litigation regarding nicotine addiction.

Acknowledgments

We thank Faith Allen, Sandy Tinetti, and Katherine Dains for assistance in data management, Lisa Yu and Tricia Mao for assistance with laboratory analysis, and Marc Olmsted for editorial assistance. We thank Dr. Saul Shiffman who conducted a study of daily and occasional smokers and provided urine samples for our analysis.

Grant Support: Research supported by NIH grants NCI CA78603, P30 DA12393, and NIH R01 HL677438 (M.D. Eisner), as well as the Flight Attendants Medical Research Institute, K24 HL 097245 (M.D. Eisner), R25CA113710, and Medical University of Silesia KNW-2-185/09 (W. Zielinska-Danch and A. Sobczak), and KNW-2-185/09 (B. Koszowski).

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

  • Received June 3, 2010.
  • Revision received August 3, 2010.
  • Accepted August 16, 2010.

References

  1. ↵
    1. Benowitz NL
    . Cotinine as a biomarker of environmental tobacco smoke exposure. Epidemiol Rev 1996;18:188–204.
    OpenUrlFREE Full Text
  2. ↵
    1. Benowitz NL,
    2. Hukkanen J,
    3. Jacob P III.
    . Nicotine chemistry, metabolism, kinetics and biomarkers. Handb Exp Pharmacol 2009:29–60.
  3. ↵
    1. Hecht SS
    . Tobacco smoke carcinogens and lung cancer. J Natl Cancer Inst 1999;91:1194–210.
    OpenUrlAbstract/FREE Full Text
    1. Boffetta P,
    2. Clark S,
    3. Shen M,
    4. Gislefoss R,
    5. Peto R,
    6. Andersen A
    . Serum cotinine level as predictor of lung cancer risk. Cancer Epidemiol Biomarkers Prev 2006;15:1184–8.
    OpenUrlAbstract/FREE Full Text
    1. Strachan DP,
    2. Jarvis MJ,
    3. Feyerabend C
    . The relationship of salivary cotinine to respiratory symptoms, spirometry, and exercise-induced bronchospasm in seven-year-old children. Am Rev Respir Dis 1990;142:147–51.
    OpenUrlPubMed
  4. ↵
    1. Whincup PH,
    2. Gilg JA,
    3. Emberson JR,
    4. et al
    . Passive smoking and risk of coronary heart disease and stroke: prospective study with cotinine measurement. BMJ 2004;329:200–5.
    OpenUrlAbstract/FREE Full Text
  5. ↵
    Department of Health and Human Services PHS. The health consequences of involuntary exposure to tobacco smoke: a report of the Surgeon General. DHHS (CDC) Publication No. 87-8398. U.S. Government Printing Office: Washington, DC; 2006.
  6. ↵
    Institute of Medicine. Secondhand smoke exposure and cardiovascular effects—making sense of the evidence. Washington, DC: National Academy Press; 2010.
  7. ↵
    1. Hecht SS
    . Biochemistry, biology, and carcinogenicity of tobacco-specific N-nitrosamines. Chem Res Toxicol 1998;11:559–603.
    OpenUrlCrossRefPubMed
  8. ↵
    1. Singer BC,
    2. Hodgson AT,
    3. Guevarra KS,
    4. Hawley EL,
    5. Nazaroff WW
    . Gas-phase organics in environmental tobacco smoke. 1. Effects of smoking rate, ventilation, and furnishing level on emission factors. Environ Sci Technol 2002;36:846–53.
    OpenUrlCrossRefPubMed
  9. ↵
    1. Schick SF,
    2. Glantz S
    . Concentrations of the carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone in sidestream cigarette smoke increase after release into indoor air: results from unpublished tobacco industry research. Cancer Epidemiol Biomarkers Prev 2007;16:1547–53.
    OpenUrlAbstract/FREE Full Text
  10. ↵
    1. Sleiman M,
    2. Gundel LA,
    3. Pankow JF,
    4. Jacob P III.,
    5. Singer BC,
    6. Destaillats H
    . Formation of carcinogens indoors by surface-mediated reactions of nicotine with nitrous acid, leading to potential thirdhand smoke hazards. Proc Natl Acad Sci U S A 2010;107:6576–81.
    OpenUrlAbstract/FREE Full Text
  11. ↵
    1. Goniewicz ML,
    2. Havel CM,
    3. Peng MW,
    4. et al
    . Elimination kinetics of the tobacco-specific biomarker and lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol. Cancer Epidemiol Biomarkers Prev 2009;18:3421–5.
    OpenUrlAbstract/FREE Full Text
  12. ↵
    1. Eisner MD,
    2. Jacob P III.,
    3. Benowitz NL,
    4. Balmes J,
    5. Blanc PD
    . Longer term exposure to secondhand smoke and health outcomes in COPD: impact of urine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol. Nicotine Tob Res 2009;11:945–53.
    OpenUrlAbstract/FREE Full Text
  13. ↵
    1. Lazcano-Ponce E,
    2. Benowitz N,
    3. Sanchez-Zamorano LM,
    4. et al
    . Secondhand smoke exposure in Mexican discotheques. Nicotine Tob Res 2007;9:1021–6.
    OpenUrlAbstract/FREE Full Text
  14. ↵
    1. Bernert JT Jr..,
    2. Turner WE,
    3. Pirkle JL,
    4. et al
    . Development and validation of sensitive method for determination of serum cotinine in smokers and nonsmokers by liquid chromatography/atmospheric pressure ionization tandem mass spectrometry. Clin Chem 1997;43:2281–91.
    OpenUrlAbstract/FREE Full Text
  15. ↵
    1. Jacob P III.,
    2. Havel C,
    3. Lee DH,
    4. Yu L,
    5. Eisner MD,
    6. Benowitz NL
    . Subpicogram per milliliter determination of the tobacco-specific carcinogen metabolite 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in human urine using liquid chromatography-tandem mass spectrometry. Anal Chem 2008;80:8115–21.
    OpenUrlCrossRefPubMed
  16. ↵
    1. Hecht SS
    . Carcinogen derived biomarkers: applications in studies of human exposure to secondhand tobacco smoke. Tob Control 2004;13 Suppl 1:i48–56.
    OpenUrlAbstract/FREE Full Text
  17. ↵
    1. Borgerding MF,
    2. Bodnar JA,
    3. Wingate DE
    . The 1999 Massachusetts Benchmark Study Final Report. A research study conducted after consultation with the Massachusetts Department of Public Health. Massachusetts: Department of Health; 2000.
  18. ↵
    1. Perez-Stable EJ,
    2. Herrera B,
    3. Jacob P III.,
    4. Benowitz NL
    . Nicotine metabolism and intake in black and white smokers. JAMA 1998;280:152–6.
    OpenUrlCrossRefPubMed
  19. ↵
    1. Richie JP Jr..,
    2. Carmella SG,
    3. Muscat JE,
    4. Scott DG,
    5. Akerkar SA,
    6. Hecht SS
    . Differences in the urinary metabolites of the tobacco-specific lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone in black and white smokers. Cancer Epidemiol Biomarkers Prev 1997;6:783–90.
    OpenUrlAbstract/FREE Full Text
  20. ↵
    1. Ashley DL,
    2. Beeson MD,
    3. Johnson DR,
    4. et al
    . Tobacco-specific nitrosamines in tobacco from U.S. brand and non-U.S. brand cigarettes. Nicotine Tob Res 2003;5:323–31.
    OpenUrlAbstract/FREE Full Text
  21. ↵
    1. Wu W,
    2. Zhang L,
    3. Jain RB,
    4. Ashley DL,
    5. Watson CH
    . Determination of carcinogenic tobacco-specific nitrosamines in mainstream smoke from U.S.-brand and non-U.S.-brand cigarettes from 14 countries. Nicotine Tob Res 2005;7:443–51.
    OpenUrlAbstract
  22. ↵
    1. Ashley DL,
    2. O'Connor RJ,
    3. Bernert JT,
    4. et al
    . Effect of differing levels of tobacco-specific nitrosamines in cigarette smoke on the levels of biomarkers in smokers. Cancer Epidemiol Biomarkers Prev 2010;19:1389–98.
    OpenUrlAbstract/FREE Full Text
  23. ↵
    1. Morse MA,
    2. Eklind KI,
    3. Toussaint M,
    4. Amin SG,
    5. Chung FL
    . Characterization of a glucuronide metabolite of 4-(methyl-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and its dose-dependent excretion in the urine of mice and rats. Carcinogenesis 1990;11:1819–23.
    OpenUrlAbstract/FREE Full Text
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Cancer Epidemiology Biomarkers & Prevention: 19 (11)
November 2010
Volume 19, Issue 11
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Urine Cotinine Underestimates Exposure to the Tobacco-Derived Lung Carcinogen 4-(Methylnitrosamino)-1-(3-Pyridyl)-1-Butanone in Passive Compared with Active Smokers
Neal Benowitz, Maciej Lukasz Goniewicz, Mark D. Eisner, Eduardo Lazcano-Ponce, Wioleta Zielinska-Danch, Bartosz Koszowski, Andrzej Sobczak, Christopher Havel and Peyton Jacob III
Cancer Epidemiol Biomarkers Prev November 1 2010 (19) (11) 2795-2800; DOI: 10.1158/1055-9965.EPI-10-0497

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Urine Cotinine Underestimates Exposure to the Tobacco-Derived Lung Carcinogen 4-(Methylnitrosamino)-1-(3-Pyridyl)-1-Butanone in Passive Compared with Active Smokers
Neal Benowitz, Maciej Lukasz Goniewicz, Mark D. Eisner, Eduardo Lazcano-Ponce, Wioleta Zielinska-Danch, Bartosz Koszowski, Andrzej Sobczak, Christopher Havel and Peyton Jacob III
Cancer Epidemiol Biomarkers Prev November 1 2010 (19) (11) 2795-2800; DOI: 10.1158/1055-9965.EPI-10-0497
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Cancer Epidemiology, Biomarkers & Prevention
eISSN: 1538-7755
ISSN: 1055-9965

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