Article Figures & Data
Figures
- Figure 1.
Whole genomic copy-number profiles of nasopharyngeal carcinoma cell lines TW01 and HONE1. The array CGH profile is shown adjacent to the ideogram of each chromosome. The results show the log2 value of the normalized signal intensity ratio from the array experiment. A log2 signal ratio of zero represents no aberrant copy number in TW01 and HONE1. Where log2 >0, it suggests amplified DNA copy-number for TW01/HONE1 relative to the reference human male genome. Where log2 <0, the reverse is inferred. Images were produced using CGH Analytics version 3.5.14 software. The array CGH data discussed in this publication have been deposited in the National Center for Biotechnology Information's Gene Expession Omnibus (50) and are accessible through GEO series accession number GSE15172 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE15172).
- Figure 2.
Gene copy number alteration as a function of gene expression in 3p12.3-p14.2, 3q26.2-q26.32, and 9p21.3-p23. According to array data, genes showing deletion and reduced expression or amplification and overexpression are indicated.
- Figure 3.
Microarray and quantitative real-time PCR cross-validated the changes of expression level of genes located on 3q26.2-q26.32 amplicon and 3p12.3-p14.2 and 9p21.3-p23 deletion regions. Stars, genes significantly up-regulated or down-regulated and chosen for further study.
- Figure 4.
FISH analysis on nasopharyngeal carcinoma tissue microarray. A. The distribution of FISH signal ratio of nasopharyngeal carcinoma tissue samples and the detection rate is indicated under each FISH probe. The definition of FISH signal ratio for gene amplification or deletion is described in Materials and Methods. B. The representative FISH images for loss of ADAMTS9 (target gene versus control gene, red signal versus green signal), LRIG1 (green versus red), ADFP (green versus red), and MDPZ (green versus red), and amplification of GPR160 (green versus red) and SKIL (red versus green).
- Figure 5.
Kaplan-Meier survival analysis for nasopharyngeal carcinoma patients. The overall survival for patients was compared based on FISH signal ratios of the defined genetic altered regions: A. 3p12.3-p14.2. B. 3q26.2-q26.32.
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Volume 18, Issue 10
