Article Figures & Data
Figures
- Figure 1.
Gene structure and primer and probe locations for the UGT2B17 multiplex real-time PCR assay. I to VI, exons of the UGT2B17 gene on the wild-type allele. The deletion allele is shown below the wild-type allele, indicating that the 120 kb are deleted including the entire UGT2B17 gene. Dashed arrows, primers that will amplify the deletion allele; dashed line (with fluorescent label Joe), deletion probe. Solid arrows, primers that will amplify exon 1 of UGT2B17 from the wild-type allele; solid line (with fluorescent label Fam), deletion probe.
- Figure 2.
Urinary NNAL glucuronidation rates grouped by UGT2B17 deletion genotype. Urinary NNAL glucuronidation rates are indicated by the ratio of NNAL-Gluc/NNAL for 82 non-Hispanic White smokers (A). Results stratified by sex are shown for 41 females (B) and 41 males (C). Points, mean; bars, SE. Significant (all subjects; *, P = 0.049) or near-significant (women only; **, P = 0.058) associations were observed comparing the NNAL-Gluc/NNAL ratios in subjects with the UGT2B17 (0/0) genotype versus subjects with the UGT2B17 (+/+) or (+/0) genotype as determined by Student's t test.
Tables
- Table 1.
Primer and probe sequences for the UGT2B17 genotype assay
Primer name Primer composition (5′-3′) Exon 1 (forward) TGAAAATGTTCGATAGATGGACATATAGTA Exon 1 (reverse) GACATCAAATTTTGACTCTTGTAGTTTTC Exon 1 (probe) 6-FAM-TACATTTTGGTCATATTTTTCACAACTACAAGAATTGT-BHQ1 Deletion (forward) TTTAATGTTTTCTGCCTTATGCCAC Deletion (reverse) AGCCTATGCAATTTTCATTCAACATAG Deletion (probe) JOE-ACTACACTGAGATTTACAAAAGAATTCTGTCAGGATATAG-BHQ1 - Table 2.
Age, sex, BMI, smoking status, education level, and case histology of 398 lung cancer cases and 697 controls
Cases [n (%)] Controls [n (%)] P Mean age* 64.1 ± 9.9 58.3 ± 10.3 <0.01 Women (%) 175 (44) 324 (46) 0.42 Mean BMI* 26.8 ± 5.0 27.3 ± 4.9 <0.01 Smoking status (%) Never smokers 35 (9) 268 (38) <0.01 Former smokers 205 (52) 291 (42) Current smokers 108 (40) 138 (20) Pack-years* 56.0 ± 38.9 23.5 ± 30.7 <0.01 Years of education (%) <High school degree 63 (16) 30 (4) <0.01 High school degree 246 (62) 392 (56) College degree 59 (15) 167 (24) Postgraduate degree 30 (8) 108 (15) Histology (%) Adenocarcinoma 151 (38) Squamous cell carcinoma 100 (27) Non–small-cell carcinoma 69 (17) Small-cell carcinoma 39 (10) Large-cell carcinoma 24 (6) Mixed histology/other 15 (4) ↵* Mean ± SD.
- Table 3.
Distribution of genotypes by sex and risk of lung cancer
UGT2B17 genotype Cases (%) Controls (%) OR (95% CI)* Men (+/+) 121 (54) 176 (47) Reference (+/0) 83 (37) 156 (42) 0.7 (0.4-1.1) (0/0) 19 (9) 41 (11) 0.7 (0.4-1.5) (+/+) + (+/0) 204 (91) 332 (89) Reference (0/0) 19 (9) 41 (11) 0.9 (0.4-1.7) Women (+/+) 91 (52) 161 (50) Reference (+/0) 58 (33) 131 (40) 0.8 (0.5-1.3) (0/0) 26 (15) 32 (10) 1.8 (0.9-3.7) (+/+) + (+/0) 149 (85) 292 (90) Reference (0/0) 26 (15) 32 (10) 2.0 (1.01-4.0) ↵* OR and 95% CI adjusted for age, BMI, education level, and pack-years.
- Table 4.
Distribution of UGT2B17 genotypes by sex and risk of lung adenocarcinoma
UGT2B17 genotype Cases (%) Controls (%) OR (95% CI)* Men (+/+) 36 (52) 176 (47) Reference (+/0) 26 (37) 156 (42) 0.7 (0.4-1.3) (0/0) 8 (11) 41 (11) 0.9 (0.4-2.3) (+/+) + (+/0) 62 (89) 332 (89) Reference (0/0) 8 (11) 41 (11) 1.0 (0.4-2.3) Women (+/+) 40 (49) 161 (50) Reference (+/0) 26 (32) 131 (40) 0.7 (0.4-1.4) (0/0) 15 (19) 32 (10) 2.4 (1.01-5.7) (+/+) + (+/0) 66 (81) 292 (90) Reference (0/0) 15 (19) 32 (10) 2.8 (1.2-6.3) ↵* OR and 95% CI adjusted for age, BMI, education level, and pack-years.
Volume 16, Issue 4
