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Cancer Epidemiology, Biomarkers & Prevention
Cancer Epidemiology, Biomarkers & Prevention
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CEBP Focus: Biorepository and Biospecimen Science

Approaches to DNA/RNA Extraction and Whole Genome Amplification

Regina M. Santella
Regina M. Santella
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DOI: 10.1158/1055-9965.EPI-06-0631 Published September 2006
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  • Table 1.

    Comparison of methods for quantitation of DNA samples

    MethodAbsorbance at 260 nmPicogreenRT-PCR SYBR green
    Sensitivity>5 μg/mL25 pg/mL-1 μg/mL<25 pg
    Type of DNA detectedSingle and double strandedDouble strandedSingle and double stranded
    Interference by RNAYesNoNo

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  • CEBP Focus: Biorepository Workshop from the AACR Annual Meeting


    The articles in this CEBP Focus section on Biorepository and Biospecimen Science were based upon a Methods Workshop session presented at the 97th AACR Annual Meeting, April 1-5, 2006, Washington, DC. Session information is as follows:

    Methods Workshop
    Sample Collection, Processing and Storage for Large Scale Studies:
    Biorepositories to Support Cancer Research

    Saturday, April 1, 2006
    2:00 PM-4:00 PM
    Room 147, Washington Convention Center
    Chairperson: Christine B. Ambrosone, Roswell Park Cancer Institute, Buffalo, NY
    Original Session Page from the 2006 AACR Annual Meeting Program

    Speakers in the Saturday Educational Program at the 2006 AACR Annual Meeting were invited to contribute state-of-the-art overviews of their critical research areas for publication in the 2006 AACR Education Book. The complete text of the Education Book is available free online at http://educationbook.aacrjournals.org.

    The CEBP Focus articles in this issue are based upon the following original overviews in the 2006 AACR Education Book:

    Establishing a Cancer Center Data Bank and Biorepository for Multidisciplinary Research
    Christine B. Ambrosone, Mary K. Nesline, and Warren Davis
    Am Assoc Cancer Res Educ Book 2006: 361-365. Ed Book Full TextEd Book PDF

    Blood: Collection, Shipment, Processing, and Storage
    Jimmie B. Vaught
    Am Assoc Cancer Res Educ Book 2006: 366-371. Ed Book Full TextEd Book PDF

    Approaches to DNA/RNA Extraction and Whole Genome Amplification
    Regina M. Santella
    Am Assoc Cancer Res Educ Book 2006: 372-376. Ed Book Full TextEd Book PDF

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    Cancer Epidemiology Biomarkers & Prevention: 15 (9)
    September 2006
    Volume 15, Issue 9
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    Approaches to DNA/RNA Extraction and Whole Genome Amplification
    Regina M. Santella
    Cancer Epidemiol Biomarkers Prev September 1 2006 (15) (9) 1585-1587; DOI: 10.1158/1055-9965.EPI-06-0631

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    Approaches to DNA/RNA Extraction and Whole Genome Amplification
    Regina M. Santella
    Cancer Epidemiol Biomarkers Prev September 1 2006 (15) (9) 1585-1587; DOI: 10.1158/1055-9965.EPI-06-0631
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      • The Preparation and Storage of DNA for Molecular Epidemiology Studies
      • Methods for Quantitation of DNA Yield and Purity
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    • Sample Collection, Processing, and Storage for Large-Scale Studies: Biorepositories to Support Cancer Research
    • Establishing a Cancer Center Data Bank and Biorepository for Multidisciplinary Research
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