Article Figures & Data
Figures
- Fig. 1.
Transcriptional response of phase 2 enzymes to sulforaphane in various prostate cancer cell lines. The cell lines LNCaP, MDA PCa 2A, MDA PCa 2B, PC3, and TSU-Pr1 were treated for 8 h with 10 μm sulforaphane or with the DMSO control. Northern blot analyses were performed using (A) NQO-1, (B) GSTA1, (C) microsomal GST, and (D) GAPDH cDNA probes.
- Fig. 2.
Northern blot analysis of the transcriptional response to sulforaphane. LNCaP cells were incubated for 1 to 72 h with 10 μm sulforaphane or the DMSO control before the RNA was harvested. The membrane was probed with labeled (A) NQO-1 and (B) GAPDH cDNAs.
- Fig. 3.
Differential effects of sulforaphane on γ-GCS subunits. Northern blot analyses of (A) sulforaphane-induced γ-GCS light chain expression over time and (B) unchanged γ-GCS heavy chain expression treated with 9 h of 10 μm sulforaphane treatment in LNCaP cells.
- Fig. 4.
QR response to sulforaphane is abolished in a reduced environment. LNCaP cells were pretreated for 2 h with 10 μm NAC followed by 8 μm sulforaphane or DMSO control before assaying QR activity. Bars, SD of triplicate data points.
Tables
- Table 1
Dose-dependent induction of quinone reductase activity in response to sulforaphane
μm sulforaphane 15 10 8 5 3 1 0.5 0.1 LNCaP 1.98 2.29 1.93 2.11 1.39 1.28 1.11 1.00 MDA Pca 2a 1.67 1.60 2.10 1.99 1.89 1.31 0.94 1.04 MDA Pca 2b 1.52 2.47 2.10 1.92 1.90 1.70 1.25 1.04 TSU-Pr1 1.86 1.28 1.39 1.14 1.21 1.01 0.95 0.92 Normal strain 1.81 1.86 1.80 1.95 2.46 2.08 1.57 1.35 - Table 2
Dose-dependent induction of quinone reductase activity in response to broccoli sprout extract
Percentage of broccoli sprout extract 1.250 0.625 0.313 0.156 0.078 0.039 0.020 LNCaP 1.138 1.974 2.134 1.462 1.176 1.044 0.957 - Table 3
Dose-dependent induction of cellular glutathione by sulforaphane
μm sulforaphane 15 10 8 5 3 1 0.5 0.1 LNCaP 1.103 1.179 1.152 1.172 1.122 1.051 1.034 1.009
Volume 10, Issue 9
