Table 1.

Univariate Cox regression analysis of relapse-free survival in patients after radical prostatectomy

VariableRelative risk (95% confidence interval)P
Age at diagnosis (n = 279)1.00 (0.97-1.03)0.881
Clinical stage (n = 278)*1.40 (0.93-2.11)0.114
Pathologic stage (n = 279)2.59 (1.75-3.84)<0.0001
Preoperative PSA (n = 263)1.78 (1.22-2.62)0.003
Gleason score (n = 279)§2.58 (1.76-3.80)<0.0001
Margins (n = 279)1.82 (1.24-2.68)0.002
Seminal vesicle involvement (n = 279)2.24 (1.52-3.30)<0.0001
Extracapsular extension (n = 279)2.30 (1.56-3.39)<0.0001
Ki67 (n = 277)1.08 (1.01-1.15)0.011
Ki67 (n = 277)1.94 (1.22-3.07)0.005
H3K18Ac POS (n = 279)**1.01 (1.00-1.02)0.043
H3K18Ac POS (n = 279)††1.73 (1.13-2.64)0.012
H3K18Ac MIOD (n = 279)‡‡1.02 (0.99-1.04)0.167
H3K18Ac MOD (n = 279)§§1.00 (0.98-1.02)0.734
H3K4diMe POS (n = 279)∥∥1.00 (0.99-1.01)0.939
H3K4diMe MIOD (n = 279)¶¶1.01 (0.99-1.02)0.443
H3K4diMe MOD (n = 279)***1.02 (1.00-1.03)0.051
H3K4diMe MOD (n = 279)†††1.56 (1.24-2.66)0.019

NOTE: All statistical analyses (footnotes ∥ to †††) were done with the highest core value present for each cancer case.

  • *Clinical stage cT1 and cT2 (also includes 7 cases cT3).

  • Pathologic stage pT2 and pT3.

  • Gleason score <7 vs. ≥ 7.

  • §Preoperative serum PSA level (ng/mL) dichotomized by cutpoint <10.0 vs. ≥10.0.

  • Ki67 (% positive cells) as a continuous variable.

  • Ki67 (% positive cells) dichotomized by low (<6% positive cells) vs. high (≥6% positive cells).

  • **H3K18Ac level (% positive nuclear area) as a continuous variable as measured by VIA.

  • ††H3K18Ac level (% positive nuclear area) dichotomized by low (<50% nuclear area) vs. high (≥50% nuclear area) as measured by VIA.

  • ‡‡H3K18Ac MIOD total amount of staining as a continuous variable as measured by VIA.

  • §§H3K18Ac MOD intensity of staining as a continuous variable as measured by VIA.

  • ∥∥H3K4diMe level (% positive nuclear area) as a continuous variable as measured by VIA.

  • ¶¶H3K4diMe MIOD total amount of staining as a continuous variable as measured by VIA.

  • ***H3K4diMe MOD intensity of staining as a continuous variable as measured by VIA.

  • †††H3K4diMe MOD intensity of staining dichotomized by low (<30 MOD) vs. high (≥30 MOD) as measured by VIA.