Table 1.

PCR amplification primers and restriction enzymes used for genotyping six single-nucleotide polymorphisms of the 1p12 region

Single-nucleotide polymorphismPrimer sequenceProduct size (bp)Restriction enzymeSize of fragments (bp)
rs4659200F 5′-TGATTCCAGCCTCTCATTAG-3′374AflIII(T)173 + 201
R 5′-GTGTTACAGCCAATGGAGTG-3′
rs3765945F 5′-CAATACCTCTAGGCTGAGCA-3′315SapI(C)153 + 162
R 5′-CAAAACAATTCCCTGTCCTC-3′
rs2145418F 5′-GAATGGCTGGTGAGGAAT-3′657AcsI(T)168 + 489
R 5′-TGTTCATTGCAGCACTATTC-3′
rs7515409F 5′-GGCTGATTAGCTCCAAGTTC-3′536TaqI(G)240 + 297
R 5′-GCATACTCCTCAGTGGCAGT-3′
rs4658973F 5′-GGGACACTTGAGACCAAAAG-3′341BsrI(T)142 + 199
R 5′-TCAAATGGGATTACAAACCT-3′
rs1241F 5′-AGCCACACTGGTAATTGATA-3′415NlaIII(C)170 + 245
R 5′-TTACAACCTGGAGATAGCTG-3′
  • Abbreviations: F, forward; R, reverse.