Table 1.

MS-PCR primer sequences and reaction conditions

Sequences1× Buffer (and additives)Annealing temperature (°C)
M3S 5′-GGTTAGTAGTTCGGGTAGGGTTTATC-3′, AS 5′-CCGAATCCTACCCCGACG-3′16.6 mmol/L (NH4)2SO457
67 mmol/L Tris (pH 9.1)
3.0 mmol/L MgCl2
U3S 5′-TTAGTAGTTTGGGTAGGGTTTATT-3′, AS 5′-CCAAATCCTACCCCAACA-3′15 mmol/L (NH4)2SO457
60 mmol/L Tris (pH 8.5)
4.5 mmol/L MgCl2
M4S 5′-GTCGAGAACGCGAGCGATTC-3′, AS 5′-CGACCAATCCAACCGAAACG-3′15 mmol/L (NH4)2SO455
60 mmol/L Tris (pH 9.0)
3.5 mmol/L MgCl2
150 mmol/L 2-pyrrolidinone
U4S 5′-GATGTTGAGAATGTGAGTGATTT-3′, AS 5′-AACCAATCCAACCAAAACA-3′15 mmol/L (NH4)2SO457
60 mmol/L Tris (pH 8.5)
4.5 mmol/L MgCl2
  • Abbreviations: S, sense; AS, antisense.