CEBP CTRC-AACR San Antonio Breast Cancer Symposium Cancer Health Disparities Conference 2009
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Madeleine, M. M.
Right arrow Articles by McDougall, J. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Madeleine, M. M.
Right arrow Articles by McDougall, J. K.
Cancer Epidemiology Biomarkers & Prevention Vol. 9, 225-227, February 2000
© 2000 American Association for Cancer Research

Short Communications

The p53 Arg72Pro Polymorphism, Human Papillomavirus, and Invasive Squamous Cell Cervical Cancer1

Margaret M. Madeleine2, Katherine Shera, Stephen M. Schwartz, Janet R. Daling, Denise A Galloway, Gregory C. Wipf, Joseph J. Carter, Barbara McKnight and James K. McDougall

Programs in Epidemiology [M. M. M., S. M. S., J. R. D.], and Cancer Biology, [K. S., D. A. G., G. C. W., J. J. C., J. K. M.], Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024; Departments of Epidemiology [S. M. S., J. R. D.] and Biostatistics, [B. M.], School of Public Health and Community Medicine, University of Washington, Seattle, Washington 98195; and Departments of Microbiology [D. A. G.], and Pathology, [J. K. M.], School of Medicine, University of Washington, Seattle, Washington 98195


   Abstract
Top
 Abstract
Introduction
 Materials and Methods
 Results
 Discussion
 References
 
A. Storey et al. [Nature (Lond.), 393: 229–234, 1998)] reported a 7-fold increased risk of cervical cancer associated with having an Arg/Arg polymorphism at codon 72 of p53 compared with the Pro/Arg heterozygotes (odds ratio, 7.4; 95% confidence interval, 2.1–29.4). Complementary in vitro studies suggested that the HPV E6 oncoprotein more readily targets the arginine form, as opposed to the proline form, of p53 for degradation. We investigated the impact of this polymorphism in a population-based case-control study of invasive cervical cancer. Using a PCR assay to detect the p53 codon 72 polymorphism, we tested blood samples from 111 women with invasive squamous cell cancer of the cervix identified by a population-based registry and 164 random-digit telephone-dialed controls. The distribution of the genotype among control women was 38% heterozygous, 7% proline homozygous, and 55% arginine homozygous, and among the cases was 38%, 6%, and 56%, respectively. There was no increased risk of squamous cell invasive cervical cancer associated with homozygosity for the arginine allele (odds ratio, 1.0; 95% confidence interval, 0.6–1.7). Furthermore, there was no modification of this result by human papillomavirus (HPV) DNA status of the tumor, age, or smoking status. Among controls, there was no association between the polymorphism and HPV-16 L1 seropositivity. However, among case subjects, the codon 72 polymorphism may be related to HPV 16L1 seropositivity status.


   Introduction
Top
 Abstract
 Introduction
Materials and Methods
 Results
 Discussion
 References
 
HPV3 is almost certainly the primary etiologic agent of cervical cancer, yet few women infected with HPV go on to develop cancer. In a recent report, Storey et al. (1) describe a 7-fold increased risk of cervical cancer associated with the p53 Arg72Pro polymorphism. Complementary in vitro studies suggested that the HPV E6 oncoprotein, which binds p53 (2) and promotes its degradation (3) , might more readily target the arginine form as opposed to the proline form of p53 for degradation. We investigated the association between the p53 Arg72Pro polymorphism and the risk of invasive squamous cell cancer of the cervix in a sample of participants from a large population-based case-control study.


   Materials and Methods
Top
 Abstract
 Introduction
 Materials and Methods
Results
 Discussion
 References
 
The methods of the larger study, described in detail in Daling et al. (4) , are briefly outlined here. Case subjects were identified by the Cancer Surveillance System, a population-based cancer registry serving western Washington State that is part of the SEER Program. The SEER Program is part of the United States National Cancer Institute and has monitored cancer incidence and mortality in a 10% sample of the United States population in nine geographic areas since 1973. Population-based control subjects were identified by using random-digit dialing and were frequency matched to case subjects by age. All of the subjects were residents of an urban, three-county area that included Seattle. The interview response rate in the parent study was 65% for case subjects and 72% for control subjects. We restricted the present investigation to white women to reduce the potential for our results to be influenced by differences in the genotypic frequency by race. The present study represents 18.0% percent of the white cases and 15.9% of the white controls in the parent study. The mean age of cases was 43.0 and that of controls was 43.6.

Serum and buffy coat samples were collected at the in-person interview, and archival tumor tissue was obtained for case subjects. Previously published methods were used to determine HPV-16 seroprevalence by a virus-like particle ELISA (5) and HPV DNA prevalence in archival tumor tissue by PCR (4) . Among those who gave a buffy-coat sample at interview and met the study restrictions (i.e., white race for all of the subjects and squamous cell histology for case subjects), a sample was randomly chosen for DNA extraction. The present study made use of DNA extracted from peripheral leukocytes from 111 case and 164 control subjects.

The PCR assay used to detect the two codon 72 alleles of p53 was performed as described by Storey et al. (1) , with minor modifications. DNA was extracted from 1 ml of buffy coat sample using the QIAamp Blood Kit (QIAGEN, Chatsworth, CA) following the instructions provided by the manufacturer. p53 arginine and proline sequences were amplified from each DNA sample in separate reactions (30 cycles) using 30 ng of genomic DNA as template, 12.5 pmol of each primer, and 1.25 units each of Amplitaq DNA polymerase (PE Applied Biosystems, Foster City, CA) and platinum Taq antibody (Life Technologies, Gaithersburg, MD). Annealing temperatures and MgCl2 concentrations were 55°C/1.0 mM and 64°C/1.5 mM for the arginine and proline amplifications, respectively. Genomic DNA extracted from a vulvar carcinoma cell line, A431 (6) , or from the peripheral blood of an individual whose genotype was determined by sequencing served as positive controls for amplification of the p53 proline and arginine alleles, respectively.

The relative risk of cancer was estimated using the OR approximation by exponentiation of coefficients obtained from multiple logistic regression analysis using EGRET (7) . The {chi}2 test was used to compare the proportions of the p53 codon 72 genotypes between HPV-16 DNA positive cases and controls (with unknown HPV DNA status) and between HPV-16 L1 serology status of cases and controls (Epi Info; Ref. 8 ).


   Results
Top
 Abstract
 Introduction
 Materials and Methods
 Results
Discussion
 References
 
The prevalence of the homozygous arginine allele was virtually identical in case and control subjects in this population (Table 1)Citation . The genotype frequencies in both populations are in Hardy-Weinberg equilibrium. Overall, there was neither an increased risk of invasive squamous cell cervical cancer associated with the arginine homozygous allele compared with the heterozygous allele in this study (OR, 1.0; 95% CI, 0.6–1.7) nor a decreased risk associated with the proline homozygous allele (OR, 0.9; 95% CI, 0.3–2.6). Among the 45 cases positive for HPV 16 DNA, the distribution of the p53 codon 72 alleles was not different from the distribution seen among the controls ({chi}2 on 2 df, 1.3; P = 0.518). Furthermore, there was no increase in the relative risk of cervical cancer associated with arginine homozygosity as compared with heterozygosity between subgroups defined by age [<45 years (OR, 0.8; 95% CI, 0.4–1.6) and >=45 years (OR, 1.3; 95% CI, 0.6–2.8)], cigarette smoking status [never (OR, 1.2; 95% CI, 0.6–2.7), former (OR, 0.9; 95% CI, 0.3–2.4), and current (OR, 0.8; 95% CI, 0.3–2.2)], or HPV-16 L1 serology [seronegative (OR, 0.6; 95% CI, 0.3–1.3) and seropositive (OR, 1.5; 95% CI, 0.7–3.0)].


View this table:
[in this window]
[in a new window]
  		Table 1 Risk of invasive squamous cell cervical cancer associated with the p53, Arg72Pro polymorphism

 
There was a difference in the distribution of the genotypes among case subjects according to their HPV-16 L1 serological status (Table 2)Citation . Nearly 64% of seropositive case subjects were homozygous for the arginine allele compared with only 44% of the seronegative case subjects ({chi}2 on 2 df, 5.70; P = 0.058). In contrast, there was no difference in the distribution of the Arg/Arg genotype among control subjects when grouped as HPV-16 L1 seronegative or seropositive ({chi}2 on 2 df, 1.06; P = 0.588).


View this table:
[in this window]
[in a new window]
  		Table 2 Prevalence of the p53 Arg72Pro polymorphism by HPV-16 L1 serology among cases and controlsa

 

   Discussion
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
References
 
Many groups have attempted to repeat the findings of Storey et al. (1) because it was thought that the potential for increased degradation of p53 by the E6 oncoprotein might contribute substantially to the risk of progression to cervical cancer. Most subsequent reports, however, found no increased risk of cervical cancer associated with the p53 Arg72Pro polymorphism (9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19) . In these reports, the relative risk of invasive cervical cancer associated with the Arg/Arg genotype compared with the heterozygous genotype ranged from 0.7 (95% CI, 0.3–1.3; Ref. 9 ) to 1.6 (95% CI, 0.6–4.4; Ref. 15 ). The distribution of the Arg/Arg polymorphism ranged from 40% (19) to 63% (9) among control women in these studies. Specifically, the prevalence of the Arg/Arg genotype was 50% or greater in control subjects from studies conducted in the United Kingdom (9, 10, 11) , Norway (12) , Sweden (13) , the United States (14) , the Netherlands (15) , Hungary (16) , Italy (17) , Germany (18) , and in the present United States study (63%, 57%, 57%, 54%, 50%, 52%, 57%, 60%, 56%, 56%, and 56%, respectively). The distribution of the Arg/Arg genotype was less than 50% among control subjects in the Japanese (19) and Costa Rican (14) populations (36% and 45%, respectively), as well as in the initial United Kingdom report (37%; Ref. 1 ).

In the only published study to support the initial findings, Zehbe et al. (20) reported an increased risk of invasive cervical cancer associated with the Arg/Arg genotype compared with the heterozygous genotype of 3.8 (95% CI, 1.1–15.1) among Italian women and 2.5 (95% CI, 1.0–7.0) among Swedish women. The prevalence of the Arg/Arg genotype was 53% in Italian controls and 50% in Swedish controls, so that this study is consistent with the majority of findings with respect to controls. However, the prevalence of the Arg/Arg genotype among case groups in this study (79% among the Italian case group and 73% among the Swedish group), was most similar to that found in the initial study (76%). The prevalence of Arg/Arg among the cases in the negative studies ranged from 40% (19) to 68% (15) . Therefore, the findings of an increased risk of cervical cancer reported by Zehbe et al. (20) and by Storey et al. (1) may be attributable to small, nonrepresentative groups of cases and controls. Further, both of these studies used tumor tissue to examine the p53 Arg72Pro polymorphism for the case group; therefore, their results may be attributable to somatic changes in tumor tissue.

Unlike some of the previous studies (1 , 7 , 10 , 13 , 19 , 20) that relied on tumor tissue from the invasive cervical cancer cases as a source of DNA for genotyping, we used peripheral leukocytes for both case and control subjects as the DNA source. Therefore, our results can be attributed neither to somatic changes in tumor tissue nor to PCR artifacts from differential amplification of the longer proline allele from formalin-fixed tissue. This strengthens the validity of our conclusion that the p53 Arg72Pro polymorphism is not associated with the risk of cervical carcinoma in our population.

Our data suggest that the Arg/Arg polymorphism may be related to antibody response to the HPV-16 L1 protein among cervical cancer patients. Although not quite statistically significant, this difference in seroprevalence could suggest that the Arg/Arg genotype might affect the ability of the host to clear an infection with HPV-16. This finding needs to be confirmed by other epidemiological studies in well-defined populations among different racial and ethnic groups. It is unclear what the mechanism might be, although in our study and other studies, a higher seroprevalence of HPV-16 L1 has been reported in women with cancer compared with controls (21) . Overall, however, our data and those of others (7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19) do not support the hypothesis that the p53 Arg72Pro polymorphism modifies the risk of cervical cancer.


   Acknowledgments
 
We thank Mary Anne Rossing and Peggy Porter for discussions on this topic, and Elizabeth Tickman and Dawn Fitzgibbons for study management help. Thanks also to Jenn I. Koop for laboratory assistance.


   Footnotes
 
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

1 Supported by National Cancer Institute Grant 3 PO1 CA 42792 and by the Cancer Surveillance System of the Fred Hutchinson Cancer Research Center, which is funded by Contract No. NO1-CN-05230 from the Surveillance, Epidemiology and End Results (SEER) Program of the National Cancer Institute with additional support from the Fred Hutchinson Cancer Research Center. Back

2 To whom requests for reprints should be addressed, at Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N. (MP-381), P.O. Box 19024, Seattle, WA 98109-1024. Back

3 The abbreviations used are: HPV, human papillomavirus; SEER, Surveillance, Epidemiology and End Results (Program); OR, odds ratio; CI, confidence interval. Back

Received 3/29/99; revised 10/28/99; accepted 11/30/99.


   References
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 References
 

  1. Storey A., Thomas M., Kalita A., Harwood C., Gardiol D., Mantovani F., Breuer J., Leigh I. M., Matlashewski G., Banks L. Role of a p53 polymorphism in the development of human papillomavirus-associated cancer. Nature (Lond.), 393: 229-234, 1998.[Medline]
  2. Werness B. A., Levine A. J., Howley P. M. Association of human papillomavirus types 16 and 18E6 proteins with p53. Science (Washington DC), 248: 76-79, 1990.[Abstract/Free Full Text]
  3. Scheffner M., Werness B. A., Huibregtse J. M., Levine A. J., Howley P. M. The E5 oncoprotein encoded by human papillomavirus types 16 and 18 promotes the degradation of p53. Cell, 63: 1129-1136, 1990.[Medline]
  4. Daling J. R., Madeleine M. M., McKnight B., Carter J. J., Wipf G. C., Ashley R., Schwartz S. M., Beckmann A. M., Hagensee M. E., Mandelson M. T., Galloway D. A. The relationship of human papillomavirus-related cervical tumors to cigarette smoking, oral contraceptive use, and prior herpes simplex virus type 2 infection. Cancer Epidemiol. Biomark. Prev., 5: 541-548, 1996.[Abstract]
  5. Carter J. J., Koutsky L. A., Wipf G. C., Christensen N. D., Lee S. K., Kuypers J., Kiviat N., Galloway D. A. The natural history of human papillomavirus type 16 capsid antibodies among a cohort of university women. J. Infect. Dis., 174: 927-936, 1996.[Medline]
  6. Harlow E., Williamson N. M., Ralston R., Helfman D. M., Adams T. E. Molecular cloning and in vitro expression of a cDNA clone for human cellular tumor antigen p53. Mol. Cell. Biol., 5: 1601-1610, 1985.[Abstract/Free Full Text]
  7. . EGRET version 1.02.07, Cytel Software Corp. Cambridge, MA 1991.
  8. Dean A., Dean J., Coulumbier D., Brendel K., Smith D., Burton A., Dicker R., Sullivan K., Fagan R., Arner T. . Epi Info, Version 6, Centers for Disease Control Atlanta, GA 1994.
  9. Rosenthal A. N., Ryan A., Al-Jehani R. M., Storey A., Harwood C. A., Jacobs I. J. p53 codon 72 polymorphism and risk of cervical cancer in UK. Lancet, 352: 871-872, 1998.[Medline]
  10. Giannoudis A., Graham D., Southern S., Herrington C. p53 codon 72 ARG/PRO polymorphism is not related to HPV type or lesion grade in low- and high-grade squamous intra-epithelial lesions and invasive squamous carcinoma of the cervix. Int. J. Cancer, 83: 66-69, 1999.[Medline]
  11. Lanham S., Campvell I., Watt P., Gornall R. p53 polymorphism and risk of cervical cancer. Lancet, 352: 1631 1998.
  12. Helland A., Legerod A., Johnsen H., Olsen A. O., Skovlund E., Borresen-Dale A-L. "p53 polymorphism and the risk of cervical cancer" (Letter). Nature (Lond.), 396: 530-531, 1998.[Medline]
  13. Josefsson A. M., Magnusson P. K. E., Ylitalo N., Quafort-Tubbin P., Ponten J., Adami H. O., Gyllesten U. B. "p53 polymorphism and the risk of cervical cancer" (Letter). Nature (Lond.), 396: 531 1998.[Medline]
  14. Hildesheim A., Schiffman M., Brinton L. A., Fraumeni J. F., Jr., Herrero R., Bratti M. C., Schwartz P., Mortel R., Barnes W., Greenberg M., McGowan L., Scott D. R., Martin M., Herrera J. E., Carrington M. "p53 polymorphism and the risk of cervical cancer" (Letter). Nature (Lond.), 396: 531-532, 1998.
  15. Hayes V., Hofstra R., Buys H., Hollema H., van der Zee A. Homozygous arginine-72 in wild type p53 and risk of cervical cancer. Lancet, 352: 1756 1998.[Medline]
  16. Szarka K., Veress G., Konya J., Gergely L. Frequency of p53 codon 72 genotypes in human papillomavirus associated squamous intraepithelial lesions and cervical cancer. Anticancer Res., 19: 2377-2379, 1999.[Medline]
  17. Bertorelle R., Chieco-Bianchi L., Del Mistro A. Papillomavirus and p53 codon 72 polymorphism. Int. J. Cancer, 82: 616-617, 1999.[Medline]
  18. Klaes R., Ridder R., Schaefer U., Benner A., von KnebelDoeberitz M. No evidence of p53 allele-specific predisposition in human papillomavirus-associated cervical cancer. J. Mol. Med., 77: 299-302, 1999.[Medline]
  19. Minaguchi T., Kanamori Y., Matushima M., Yoshikawa H., Taketani Y., Nakamura Y. No evidence of correlation between polymorphism at codon 72 of p53 and risk of cervical cancer in Japanese patients with human papillomavirus 16/18 infection. Cancer Res., 58: 4585-4586, 1998.[Abstract/Free Full Text]
  20. Zehbe I., Voglino G., Wilander E., Genta F., Tommasino M. Codon 72 polymorphism of p53 and its association with cervical cancer. Lancet, 354: 218-219, 1999.[Medline]
  21. Carter J., Galloway D. Humoral immune response to human papillomavirus infection. Clin. Dermatol., 15: 249-259, 1997.[Medline]



This article has been cited by other articles:


Home page
 Cancer Res.Home page
M. M. Madeleine, L. G. Johnson, A. G. Smith, J. A. Hansen, B. B. Nisperos, S. Li, L.-P. Zhao, J. R. Daling, S. M. Schwartz, and D. A. Galloway
Comprehensive Analysis of HLA-A, HLA-B, HLA-C, HLA-DRB1, and HLA-DQB1 Loci and Squamous Cell Cervical Cancer Risk
Cancer Res., May 1, 2008; 68(9): 3532 - 3539.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
Y. Zhu, M. R. Spitz, C. I. Amos, J. Lin, M. B. Schabath, and X. Wu
An Evolutionary Perspective on Single-Nucleotide Polymorphism Screening in Molecular Cancer Epidemiology
Cancer Res., March 15, 2004; 64(6): 2251 - 2257.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
A. Koushik, R. W. Platt, and E. L. Franco
p53 Codon 72 Polymorphism and Cervical Neoplasia: A Meta-Analysis Review
Cancer Epidemiol. Biomarkers Prev., January 1, 2004; 13(1): 11 - 22.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
F X Bosch, A Lorincz, N Munoz, C J L M Meijer, and K V Shah
The causal relation between human papillomavirus and cervical cancer
J. Clin. Pathol., April 1, 2002; 55(4): 244 - 265.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
G. Liu, D. P. Miller, W. Zhou, S. W. Thurston, R. Fan, L.-L. Xu, T. J. Lynch, J. C. Wain, L. Su, and D. C. Christiani
Differential Association of the Codon 72 p53 and GSTM1 Polymorphisms on Histological Subtype of Non-Small Cell Lung Carcinoma
Cancer Res., December 1, 2001; 61(24): 8718 - 8722.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Madeleine, M. M.
Right arrow Articles by McDougall, J. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Madeleine, M. M.
Right arrow Articles by McDougall, J. K.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online