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Glutathione S-Transferase M1 Status and Lung Cancer Risk

A Meta-Analysis

Section of Cancer Genetics, Institute of Cancer Research, Sutton, Surrey SM2 5NG, United Kingdom

	Abstract

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Interindividual differences in lung cancer susceptibility may be mediated in part through polymorphic variability in the bioactivation of procarcinogens. GSTM1 status has been extensively studied in this context as a lung cancer risk factor, although published studies have produced conflicting results. To clarify the impact of GSTM1 status on lung cancer risk a meta-analysis of 23 case-control studies from the literature has been carried out using a random effects model. The principal outcome measure was the odds ratio for the risk of lung cancer. There was heterogeneity between the studies attributable to differences in the methods of assigning GSTM1 status. Pooling the studies that were based on phenotyping methods, the overall odds ratio of lung cancer risk associated with GSTM1 deficiency was 2.12 (95% confidence interval, 1.43–3.13). The risk of lung cancer risk associated with GSTM1 deficiency derived from the studies based on genotyping methods was, however, lower. The overall odds ratio was 1.13 (95% confidence interval, 1.04–1.25). These findings suggest that the estimates of lung cancer risk associated with GSTM1 deficiency in the early studies, based on phenotyping, were overinflated. Moreover, it is conceivable, given publication bias, that GSTM1 status has no effect on the risk of lung cancer per se. A major concern in case-control studies of polymorphisms and cancer risk is bias. A review of the 23 case-control studies indicates that greater attention should, therefore, be paid to the design of future studies.

	Introduction

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Lung cancer accounts for 19% of all cancers and 29% of all cancer deaths. It is the commonest cause of cancer death in men, and is second only to breast cancer in women (1 , 2) . It is frequently cited as an example of a malignancy solely attributable to environmental exposure to carcinogens. Undoubtedly tobacco smoking is the most important etiological factor in the development of this cancer, and the risk is at least 10 times higher in long-term smokers compared with nonsmokers (3) . However, there is a growing realization that the development of most cancers results from a complex interaction of both environmental and genetic factors. Epidemiological studies have shown that relatives of lung cancer cases are at a 2-fold elevated risk of developing the disease (4) . It is possible that part of the susceptibility to lung cancer may be determined by the interindividual difference in the bioactivation of procarcinogens and detoxification of carcinogens.

GSTM1² has been of considerable interest as a lung cancer susceptibility gene. The biochemical basis for a possible association is that GSTM1 is one of a family of glutathione S-transferases capable of detoxifying reactive electrophiles that can act as mutagens. Hence, GSTM1 may be involved in the inactivation of this class of procarcinogens. The GSTM1 gene is polymorphic, and at least four alleles exist (5) . The GSTM1*0 allele represents a deletion, and individuals homozygous for this null allele are less efficient at conjugating and detoxifying specific substrate intermediates of carcinogens (5) .

Seidegard et al. (6) first reported an association between GSTM1 deficiency and lung cancer. Since the publication of this report in 1985, over 20 studies have appeared in the literature confirming or refuting an association between GSTM1 deficiency and lung cancer risk (7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26) . One of the major problems of the published studies is that most of them have been based on only small numbers. To clarify the effect of GSTM1 status on the risk of lung cancer a meta-analysis of all of the studies published between 1985 and 1998 on the possible association between GSTM1 status and lung cancer risk has been undertaken.

	Materials and Methods

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Identification of Studies.
A search of the literature was made using two electronic databases, MEDLINE and BIDS EMBASE for the years 1985–1998 to identify articles in which the GSTM1 status was determined in lung cancer patients and controls. Additional articles were ascertained through references cited in these publications. Articles included for analyses were primary references and of case-control design. In all of the studies GSTM1 status was determined by either enzymatic assay with trans-stilbene oxide as substrate, by immunological techniques, or by analysis of the gene through Southern blotting or PCR.

Statistical Analysis.
The odds ratio of lung cancer associated with GSTM1 deficiency was estimated for each study. These odds ratios and their corresponding 95% CIs were plotted against the number of participants in each of the studies to detect any obvious sample size bias. To take into account the possibility of heterogeneity between studies, a random effects model was used for the derivation of odds ratios (27) . This model assumes that the studies in question are a random sample of a hypothetical population of studies taking into account within- and between-study variability. Statistical manipulations were undertaken using the program Meta-analyst.³ The power of each study was computed as the probability of detecting an association between GSTM1 deficiency and lung cancer at the 0.05 level of significance, assuming a genotypic risk of 1.5 and 2.0. These estimates of power were performed on the basis of the method published by Fleiss et al. (28) , using the statistical program POWER (Epicenter Software, Version 1.30).

	Results

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Twenty reports detailing 23 case-control studies of the possible association between GSTM1 status and lung cancer risk that were suitable for analysis were identified from the literature (Refs. 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 ; Table 1 ). Reasons for excluding reports from the analysis were: (a) the same data were available in more than one study; (b) no control group of individuals had been ascertained; or (c) only family members had been studied (29, 30, 31, 32, 33, 34) .

Of the reports selected for meta-analysis, 4 determined GSTM1 status by phenotyping, and in the remaining 18, GSTM1 status was based on genotypes (Table 1) . In two studies GSTM1 status had been determined by both methods but only the genotyping or phenotyping data were used in the meta-analysis to avoid counting results from these two studies twice. In the study reported by Nazar-Stewart et al. (12) , GSTM1 phenotypes of cases and controls were used because genotypes had been determined only on a subset of cases and controls that had been phenotyped. Because both genotypes and phenotypes were available on all of the cases and controls reported by Brockmuller et al. (13) , only the genotypes were used in the analysis.

Table 1 shows the power of individual studies to demonstrate an association between GSTM1 deficiency and lung cancer risk if the true risk was 1.5 or 2. A statistical power greater than 80% was attained by 11 of the 23 studies if the genotypic risk was equal to or greater than 2.0 ( = 0.05, two tails). However, if the genotypic risk was 1.5, only 2 of 23 of the studies had power greater than 80% to demonstrate an association.

Fig. 1 shows a plot of odds ratios (95% confidence limits) for the risk of developing lung cancer associated with GSTM1 deficiency in the 23 case-control studies. The median odds ratio value was greater than unity in 17 of the studies but was only statistically significant (P < 0.05) in four. A plot of GSTM1 deficiency and lung cancer risk showed a trend toward a less significant association between GSTM1 status in the larger of the studies (Fig. 1) . An impression of heterogeneity between the studies was confirmed by formal statistical analysis (² = 37.9; 22 df; P < 0.02). This heterogeneity could be attributed to the differences between studies in the methods of determining GSTM1 status. Stratifying the studies by methodology showed no evidence for heterogeneity between the three studies using phenotyping methods (² = 4.01; 3 df; P > 0.2) or those based on genotypes (² = 21.08; 18 df; P > 0.2). When the studies were pooled that were based on phenotyping methods, the overall odds ratio of lung cancer risk associated with GSTM1 deficiency was 2.54 (95% CI, 1.74–3.72; Fig. 2 ). The risk of lung cancer risk associated with GSTM1 deficiency derived from the studies based on genotyping methods was, however, lower (Fig. 3) . The overall odds ratio associated with GSTM1 deficiency determined from genotyping was 1.13 (95% CI, 1.03–1.25).

View larger version (31K): [in this window] [in a new window]   Fig. 1. All of the studies. Odds ratios and 95% CIs for the risk of developing lung cancer associated with GSTM1 deficiency. The studies are stratified by size. Summary: odds ratio 1.20 (95% CI, 1.06–1.35).

View larger version (10K): [in this window] [in a new window]   Fig. 2. Studies based on GSTM1 phenotypes. Odds ratios and 95% CIs for the risk of developing lung cancer associated with GSTM1. Summary: odds ratio, 2.12 (95% CI, 1.43–3.13).

View larger version (26K): [in this window] [in a new window]   Fig. 3. Studies based on GSTM1 genotypes. Odds ratios, 95% CIs for the risk of developing lung cancer associated with GSTM1 deficiency. Summary: odds ratio, 1.14 (95% CI, 1.03–1.25).

It is conceivable that GSTM1 deficiency may be associated with a specific form of lung cancer. Of 23 studies that have examined a relationship between GSTM1 status and lung cancer risk, 13 contain information on histology in a form suitable for a pooled analysis (6, 7, 8 , 10 , 11 , 13 , 14 , 16 , 17 , 22 , 23, 24, 25) . Of these 13, 10 determined GSTM1 status by genotyping in a form that permits a pooled analysis to be undertaken (8 , 10 , 11 , 14 , 16 , 17 , 22 , 23, 24, 25) . By pooling these studies, GSTM1 deficiency was associated with an odds ratio of 1.40 for small cell carcinoma (95% CI, 1.01–1.95), 1.26 for adenocarcinoma (95% CI, 0.97–1.64), and 1.31 for squamous carcinoma (95% CI, 1.02–1.68). Tests for heterogeneity in each of these histological subgroups analyzed showed no evidence for heterogeneity except in the squamous carcinoma group (P < 0.05).

	Discussion

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Most of the cancer susceptibility genes identified to date are rare and highly penetrant. They may cause a substantial proportion of cancers at young ages but are unlikely to make a contribution to a high percentage of all cancers. However, less penetrant genes could well do so, and there is increasing evidence for participation of this class of genes in a number of cancers (4) . Given that exposure to carcinogens is a major risk factor for lung cancer, the hypothesis that the modulation of carcinogen metabolism is under genetic control is a plausible mechanism for explaining interindividual susceptibility.

Since Seidegard et al. (6) first drew attention to a possible relationship between GSTM1 deficiency and lung cancer risk, 20 reports have been published examining this hypothesis (7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26) . All but two of these studies failed to demonstrate such a strong association between GSTM1 status and lung cancer (7 , 12) . It is not uncommon for the first published studies to report over-inflated estimates of risk or effects that subsequent studies cannot replicate. Furthermore, it is possible for negative findings to go unreported, which leads to biased conclusions.

The frequency of GSTM1 deficiency is approximately 50% in most Caucasian populations. If deficiency is associated with a 1.5-fold increase in lung cancer risk, most of the published studies have very limited power to demonstrate such a moderate effect. McWilliams et al. in 1995 (35) pooled 11 of the then available case-control studies and concluded that GSTM1 deficiency conferred a 1.4-fold increase in the risk of lung cancer (95% CI, 1.2–1.6). This overview included the studies based on phenotyping as an analytical method to establish GSTM1 status. Although studies have shown that GSTM1 phenotypes and genotypes are highly correlated, the concordance between results is not absolute (coefficient of association, = 0.85–0.97; Refs. 12 , 13 ). Misclassification of GSTM1 status on the basis of phenotypes is, therefore, a distinct possibility in some studies. This, coupled with the small sample sizes of many of the early reports makes the results of such studies capricious. Although it is conceivable that a rare variant of GSTM1 may not be detected using PCR-based methods, genotypes are very unlikely to be prone to as many misclassifications as phenotyping methods.

Since the overview by McWilliams et al. in 1995 (35) , a large number of studies have reported on the possible association between GSTM1 status and lung cancer risk. All of these have assigned GSTM1 status on the basis of genotyping and most were based on larger sample sizes than the reports published before 1995. The continuing debate about the possible role of GSTM1 deficiency as a lung cancer risk factor and the fact that 20 studies based on genotyping have been published prompted the present meta-analysis to derive an estimate of the risk associated with GSTM1 status.

In this meta-analysis, only published studies were used. Publication bias is, therefore, an issue. Ideally, quality scoring of studies should be used to determine which are to be included in any meta-analyses (36) . This was not undertaken because the existing scales have not been validated, and it is also unclear how they could be readily applied to the published cases-control studies on GSTM1 status and lung cancer (37) . It is, however, clear that some of the studies are far from perfect in design. The issue of false-positive findings in association studies is a great concern. Any stratification within a population sample can lead to spurious evidence for an association between the marker and the disease. To avoid this problem, the identification is required of subpopulations defined in terms of factors influencing disease and marker-allele frequencies. These include ethnicity and geographical origin. In a number of the studies, the ethnicity of cases and controls were probably mixed. The frequency of GSTM1 deficiency varies considerably between ethnic groups; therefore, a failure to match cases and controls represents a source of bias. Furthermore, several GSTM genes are localized in a cluster on chromosome 1p; therefore, it is possible that an association mediated by linkage disequilibrium may be confined to certain populations. Hence population stratification would mask such an effect.

Wolf et al. (38) have suggested that case-control protocols are unnecessary in genotyping studies because age, smoking, and concomitant disease do not influence genotype. The age of cases and controls is relevant, however, because the frequency of genotypes may display age-dependency. For example, it has been shown that the population frequency of ApoE polymorphisms, which influence circulating lipoprotein levels, show age-dependency (39) . Furthermore, age is relevant to determining the probable exposure to carcinogens. A difference in the ages between cases and controls is, therefore, a potential source of bias. Even though adjustment for these covariates can be made using logistic regression, many studies are small, and adjustment may not be adequately achieved.

If genetic susceptibility to lung cancer is in part mediated through polymorphic variation, it is probable that the risk associated with any one locus will be small because a multiplicative model of interaction is likely to operate. Hence, combinations of certain genotypes may be more discriminating as risk factors than a single locus genotype. There is some support for this hypothesis in that both GSTM1 and GSTP1 enzymes affect the biotransformation of certain mutagens (40) .

Considerable effort and resources have been put into testing possible associations between metabolic polymorphisms and cancer risk, but there are serious errors inherent in the design of some published studies. It is clear that in addition to basing studies on sample sizes commensurate with the detection of low-penetrance genes, more attention should be paid to adequate matching of cases and controls to avoid the potential problems of population stratification and other sources of bias.

The findings of this meta-analysis suggest that the estimates of lung cancer risk associated with GSTM1 deficiency in the early studies, based on phenotyping, were exaggerated. Furthermore, results from pooling the studies in which GSTM1 status was derived from genotyping alone makes it conceivable that GSTM1 status has no effect on the risk of lung cancer. This finding is perhaps not surprising because the a priori evidence to support the role of GSTM1 status as a lung cancer risk factor is not strong. Although the enzyme catalyzes the detoxification of polyaromatic hydrocarbons in vitro, expression of GSTM1 in lung tissue is very low (41 , 42) . Given that the primary site for tissue expression of GSTM1 is the liver, any increased lung cancer risk directly associated with a lack of GSTM1 activity would, therefore, have to be mediated by blood-borne metabolites from the hepatic system (43) .

	Acknowledgments

	Footnotes

 
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom requests for reprints should be addressed, at Section of Cancer Genetics, Institute of Cancer Research, Haddow Laboratories, 15 Cotswold Road, Sutton, Surrey SM2 5NG, United Kingdom. Phone: 44-0-181-643-8901; Fax: 44-0-181-643-0257; E-mail: r.houlston{at}icr.ac.uk

² The abbreviations used are: GSTM1, glutathione S-transferase M1; CI, confidence interval.

³ I. Lau and T. C. Chalmers; <http://www.familypractice.msu.edu>.

Received 1/12/99; revised 5/21/99; accepted 5/25/99.

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				C. M. McBride, I. M. Lipkus, D. Jolly, and P. Lyna Interest in Testing for Genetic Susceptibility to Lung Cancer among Black College Students "At Risk" of Becoming Cigarette Smokers Cancer Epidemiol. Biomarkers Prev., December 1, 2005; 14(12): 2978 - 2981. [Abstract] [Full Text] [PDF]

				S. C. Sanderson and J. Wardle Will Genetic Testing for Complex Diseases Increase Motivation to Quit Smoking? Anticipated Reactions in a Survey of Smokers Health Educ Behav, October 1, 2005; 32(5): 640 - 653. [Abstract] [PDF]

				R. Lai, L. Crevier, and L. Thabane Genetic Polymorphisms of Glutathione S-Transferases and the Risk of Adult Brain Tumors: A Meta-analysis Cancer Epidemiol. Biomarkers Prev., July 1, 2005; 14(7): 1784 - 1790. [Abstract] [Full Text] [PDF]

				J. S. Barnholtz-Sloan, R. Chakraborty, T. A. Sellers, and A. G. Schwartz Examining Population Stratification via Individual Ancestry Estimates versus Self-Reported Race Cancer Epidemiol. Biomarkers Prev., June 1, 2005; 14(6): 1545 - 1551. [Abstract] [Full Text] [PDF]

				M. J. Kelley, E. M. Glaser, J. E. Herndon II, F. Becker, R. Bhagat, Y.-J. Zhang, R. M. Santella, S. G. Carmella, S. S. Hecht, L. Gallot, et al. Safety and Efficacy of Weekly Oral Oltipraz in Chronic Smokers Cancer Epidemiol. Biomarkers Prev., April 1, 2005; 14(4): 892 - 899. [Abstract] [Full Text] [PDF]

				M.L. Cote, S.L.R. Kardia, A.S. Wenzlaff, S.J. Land, and A.G. Schwartz Combinations of glutathione S-transferase genotypes and risk of early-onset lung cancer in Caucasians and African Americans: a population-based study Carcinogenesis, April 1, 2005; 26(4): 811 - 819. [Abstract] [Full Text] [PDF]

				Y. Hu, M. P. McDermott, and S. A. Ahrendt The p53 Codon 72 Proline Allele Is Associated with p53 Gene Mutations in Non-Small Cell Lung Cancer Clin. Cancer Res., April 1, 2005; 11(7): 2502 - 2509. [Abstract] [Full Text] [PDF]

				M. R. Spitz, X. Wu, and G. Mills Integrative Epidemiology: From Risk Assessment to Outcome Prediction J. Clin. Oncol., January 10, 2005; 23(2): 267 - 275. [Abstract] [Full Text] [PDF]

				A.-K. Alexandrie, F. Nyberg, M. Warholm, and A. Rannug Influence of CYP1A1, GSTM1, GSTT1, and NQO1 Genotypes and Cumulative Smoking Dose on Lung Cancer Risk in a Swedish Population Cancer Epidemiol. Biomarkers Prev., June 1, 2004; 13(6): 908 - 914. [Abstract] [Full Text] [PDF]

				G. Liang, X. Miao, Y. Zhou, W. Tan, and D. Lin A functional polymorphism in the SULT1A1 gene (G638A) is associated with risk of lung cancer in relation to tobacco smoking Carcinogenesis, May 1, 2004; 25(5): 773 - 778. [Abstract] [Full Text] [PDF]

				A. G. Schwartz Genetic Predisposition to Lung Cancer Chest, May 1, 2004; 125(5_suppl): 86S - 89S. [Full Text] [PDF]

				S. S. Hecht, M. Chen, H. Yagi, D. M. Jerina, and S. G. Carmella r-1,t-2,3,c-4-Tetrahydroxy-1,2,3,4-tetrahydrophenanthrene in Human Urine: A Potential Biomarker for Assessing Polycyclic Aromatic Hydrocarbon Metabolic Activation Cancer Epidemiol. Biomarkers Prev., December 1, 2003; 12(12): 1501 - 1508. [Abstract] [Full Text] [PDF]

				C. Sweeney, V. Nazar-Stewart, P. L. Stapleton, D. L. Eaton, and T. L. Vaughan Glutathione S-transferase M1, T1, and P1 Polymorphisms and Survival among Lung Cancer Patients Cancer Epidemiol. Biomarkers Prev., June 1, 2003; 12(6): 527 - 533. [Abstract] [Full Text] [PDF]

				P. Wallstrom, K. Frenkel, E. Wirfalt, B. Gullberg, J. Karkoszka, J. Seidegard, L. Janzon, and G. Berglund Antibodies against 5-Hydroxymethyl-2'-deoxyuridine Are Associated with Lifestyle Factors and GSTM1 Genotype: A Report from the Malmo Diet and Cancer Cohort Cancer Epidemiol. Biomarkers Prev., May 1, 2003; 12(5): 444 - 451. [Abstract] [Full Text] [PDF]

				N. E. Caporaso Why Have We Failed to Find the Low Penetrance Genetic Constituents of Common Cancers? Cancer Epidemiol. Biomarkers Prev., December 1, 2002; 11(12): 1544 - 1549. [Full Text] [PDF]

				I. Stucker, A. Hirvonen, I. de Waziers, A. Cabelguenne, K. Mitrunen, S. Cenee, E. Koum-Besson, D. Hemon, P. Beaune, and M.-A. Loriot Genetic polymorphisms of glutathione S-transferases as modulators of lung cancer susceptibility Carcinogenesis, September 1, 2002; 23(9): 1475 - 1481. [Abstract] [Full Text] [PDF]

				S. Benhamou, W. J. Lee, A.-K. Alexandrie, P. Boffetta, C. Bouchardy, D. Butkiewicz, J. Brockmoller, M. L. Clapper, A. Daly, V. Dolzan, et al. Meta- and pooled analyses of the effects of glutathione S-transferase M1 polymorphisms and smoking on lung cancer risk Carcinogenesis, August 1, 2002; 23(8): 1343 - 1350. [Abstract] [Full Text] [PDF]

				F. D. Gilliland, H. J. Harms, R. E. Crowell, Y.-F. Li, R. Willink, and S. A. Belinsky Glutathione S-Transferase P1 and NADPH Quinone Oxidoreductase Polymorphisms Are Associated with Aberrant Promoter Methylation of P16INK4a and O6-Methylguanine-DNA Methyltransferase in Sputum Cancer Res., April 1, 2002; 62(8): 2248 - 2252. [Abstract] [Full Text] [PDF]

				I. Stucker, P. Boffetta, S. Antilla, S. Benhamou, A. Hirvonen, S. London, and E. Taioli Lack of Interaction between Asbestos Exposure and Glutathione S-Transferase M1 and T1 Genotypes in Lung Carcinogenesis Cancer Epidemiol. Biomarkers Prev., December 1, 2001; 10(12): 1253 - 1258. [Abstract] [Full Text] [PDF]

				B. Zhao, A. Seow, E. J. D. Lee, W.-T. Poh, M. Teh, P. Eng, Y.-T. Wang, W.-C. Tan, M. C. Yu, and H.-P. Lee Dietary Isothiocyanates, Glutathione S-transferase -M1, -T1 Polymorphisms and Lung Cancer Risk among Chinese Women in Singapore Cancer Epidemiol. Biomarkers Prev., October 1, 2001; 10(10): 1063 - 1067. [Abstract] [Full Text] [PDF]

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