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hOGG1 Ser326Cys Polymorphism and Lung Cancer Susceptibility¹

Department of Pathology, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan [H. S., K. N., H. I., S. B., K. S.]; Biology Division, National Cancer Center Research Institute, Tokyo 104-0045, Japan [T. K., K. S., J. Y.]; Department of Preventive Medicine, Nagoya University School of Medicine, Nagoya 466-8550, Japan [K. W., Y. O.]; National Okinawa Hospital, Okinawa 901-2214, Japan [K. G.]; Hypertension Gene Laboratory and Institute for Biomedical Research, Department of Physiology, The University of Sydney, Sydney, New South Wales 2006, Australia [B. J. M.]; Cancer Institute of Jiangsu Province, Nanjing, Jiangsu, People’s Republic of China [C. G., Z. L., J. W.]; Epidemiology Division, Aichi Cancer Center Research Institute, Nagoya 464-8681, Japan [T. T., K. T.]; Department of Forensic Medicine, Albert Szent Gyorgyi Medical University, Kossuth L.sg1.40, Hungary [T. V.]; Department of Legal Medicine, Tokyo Women’s Medical College, Tokyo 162-8666, Japan [T. S.]; The Institute of Statistical Mathematics, Tokyo 106-8569, Japan [J. K. L.]; Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania 15261 [J. J. M.]; Epidemiology and Biostatistics Division, National Cancer Center Research Institute East, Chiba 277-8577, Japan [S. T.]; and Department of Pathology, Ryukyu University School of Medicine, Okinawa 903-0125, Japan [T. I.]

	Abstract

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The human homologue of the yeast OGG1 gene, hOGG1, has been cloned, and its genetic structure has been determined. Several polymorphisms in the hOGG1 gene were detected in the Japanese populations, and among them, the Ser-Cys polymorphism at codon 326 has been shown to have a functional difference in complementation of mutant Escherichia coli that is defective in the repair of 8-hydroxyguanine. Activity in the repair of 8-hydroxyguanine is greater in hOGG1-Ser³²⁶ protein than in hOGG1³²⁶ protein. Because many environmental carcinogens produce 8-hydroxyguanine residue and mismatching to this modified base potentially causes oncogenic mutations, the capacity to repair these lesions can be involved in cancer susceptibility in human beings. We, therefore, examined allele distributions of the Ser326Cys polymorphism in a case-control study of male lung cancer in Okinawa. The analyses based on 241 cases and 197 hospital controls disclosed the following findings. (a) Those with the Cys/Cys genotype were at an increased risk of squamous cell carcinoma and nonadenocarcinoma compared to those with the Ser/Cys and those with the Ser/Ser genotypes combined. The odds ratios adjusted for age and smoking history were 3.01 (95% confidence interval, 1.33–6.83) and 2.18 (95% confidence interval, 1.05–4.54), respectively. (b) The odds ratios for other histological subtypes of lung cancer or those in total were not significant. Those for Cys/Cys or Ser/Cys genotype against Ser/Ser did not reach statistical significance in any cell type. (c) The distributions of this polymorphism varied for different populations (Chinese, Japanese, Micronesians, Melanesians, Hungarians, and Australian Caucasians), with much less prevalence of Cys allele in the latter three populations. Although our sample size was limited, these results indicate that the Ser326Cys variant may be related to squamous cell lung cancer susceptibility. The Cys/Cys genotype appears to be more susceptible to squamous cell carcinoma, although the risk is less than that previously reported to be associated with the CYPIA1 gene. Further studies are needed to assess the importance of the interpopulation variation to cancer susceptibility.

	Introduction

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Molecular cloning of a human counterpart of the yeast OGG1 (hOGG1; Refs. 1, 2, 3, 4, 5, 6 ) paved the way for possible application of hOGG1 variants as genetic markers for individual susceptibility to various cancers. This is because 8-hydroxyguanine, assumed to be repaired by hOGG1, is highly mutagenic, causing a major oxidative DNA damage induced by reactive oxygen species (7 , 8) . Moreover, the content of 8-hydroxyguanine in DNA of nontumor lung tissue has been shown to be higher in lung cancer patients than in controls (9) . Our previous structural studies as well as those by others revealed the presence of several polymorphisms inside hOGG1 in the Japanese population (10, 11, 12) . Among them, the polymorphism Ser326Cys is associated with complementation activity for Escherichia coli mutants that are defective in the repair of 8-hydroxyguanine (10) . On the basis of our previous studies, the capacity to complement is 7 times higher in the 326Ser protein than in the 326Cys protein, suggesting that 326Cys allele may give an individual more susceptibility to the formation of 8-hydroxyguanine in DNA (10 , 11) . Because previous studies suggest a role of oxidative DNA damage in lung carcinogenesis (3) , we evaluated this presumably functional polymorphism of hOGG1 in a case-control framework of human lung cancer.

	Subjects and Methods

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Case-Control DNA Set.
Samples of DNA from lung cancer patients and hospital controls used here were the same as those used in our previous case-control study for CYPIA1 (13) . Briefly, all lung cancer patients and hospital controls were males, recruited from the National Okinawa Hospital, whose diagnoses of lung cancer were verified histopathologically and cytologically. Histological categorization was performed based on the WHO criteria (14 , 15) . DNA was available from 241 cases and 197 hospital controls. The cases constituted a sequential series of newly diagnosed lung cancer patients at the National Okinawa Hospital from 1993 to 1996. In our previous study (16) , we showed that lung cancer patients in this hospital could represent lung cancer in Okinawa. Controls were randomly selected from in-patients at the hospital during the same study period. The participation rate was nearly 100% in both cases and controls, and only six cases were excluded from the final analysis because their diagnoses were not pathologically confirmed. The diagnoses of the controls included 19 tuberculoses, 8 cancers outside the lung, 8 benign tumors, 15 bronchopneumonia, 8 pyothorax, 7 chronic obstructive lung diseases, 29 other respiratory diseases, 13 benign liver diseases, 15 other gastrointestinal diseases, 13 diabetes, 9 fractures, 15 other orthopedic diseases, 25 neuromuscular diseases, 11 others, and 2 healthy individuals. Smoking history was obtained via interviews by doctors and nurses and was reviewed by the researchers. Smoking variables such as starting and quitting ages of smoking and numbers of cigarettes per day were also collected.

PCR-SSCP³ Analysis.
The genomic structure of hOGG1 is shown in Fig. 1 . The primers used for identification of the Ser326Cys polymorphism in exon 7 were 5'-TGAATTCGGAAGGTGCTTGGGGAAT-3' and 5'-ACTGTCACTAGTC-TCACCAG-3'. A forward primer, 5'-GGAAGGTGCTTGGGGAAT-3', without an EcoRI restriction site was also used for the same DNA in combination with the same backward primer, 5'-ACTGTCACTAGTCTCACCAG-3'. The PCR product was denatured with formamide at 95°C for 15 min, quenched on ice, and loaded to polyacrylamide gels under several conditions. Visualization was performed with a silver stain kit (Wako, Osaka, Japan) as described previously (17) . Unambiguous migration differences between the two allelic bands were obtained by electrophoresis performed at room temperature and 4°C with a glycerol concentration of 12%. In addition, each allelic PCR product was cloned and sequenced to confirm the genotypes inferred from SSCP analysis. Sequencing involved a dye-primer cycle sequence kit (Amersham, Cleveland, OH) and an ABI 301 automated sequencer (Applied Biosystems, Foster City, CA).

View larger version (6K): [in this window] [in a new window]   Fig. 1. Genomic structure of hOGG1 showing previously identified polymorphic sites (10 , 33 , 38) . The Ser-Cys polymorphism is in exon 7 (C/G) and in linkage disequilibrium with the polymorphism in intron 4 (C/G; Ref. 10 ).

Population Study.
Six populations that were free from cancer were studied to detect a difference in the distributions of the polymorphism among populations: Chinese in Jiangsu Province, Australian Caucasians, Hungarians (21) , Japanese in Tokyo, Micronesians from the Republic of Palau and Yap State, and Melanesians from three islands of Vanuatu (22 , 23) . All were randomly sampled, unrelated individuals. Differences in the distributions among the populations were tested by the ² test. Deviation from the Hardy-Weinberg equilibrium was also tested (19) .

	Results

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Identification of the Ser326Cys Polymorphism by Non-Radioisotope SSCP.
Electrophoresis at room temperature with 12% glycerol discriminated three genotypes, as shown in Fig. 2a . Migration patterns were different when the primers without restriction enzyme site tags were used, but the genotypes assigned were consistent (Fig. 2a) . Confirmatory sequence results are shown in Fig. 2b .

View larger version (36K): [in this window] [in a new window]   Fig. 2. a, three genotypes revealed by SSCP analysis in 12% polyacrylamide gels. Lanes 1, 3, 4, and 5, Ser/Cys; Lane 2, Cys/Cys; and Lane 6, Ser/Ser. Right, PCR products with EcoRI tag primers. b, confirmatory base substitution identification by sequencing. Differences (underlined) in a base G (top, Cys allele) and C (bottom, showing complimentary sequence, Ser allele) at codon 326 are indicated.

ORs by Smoking Dosage.
The ORs by smoking dosage are shown in Tables 2 and 3 . The OR for Cys/Cys genotype versus either the Ser/Ser homozygote or the two other genotypes combined was greater in heavy smokers (cigarette-years 800) but was not statistically significant. Further adjustment for smoking-years in each strata did not modify the ORs substantially (data not shown).

Statistical Power.
Our power calculations indicated that the analyses had the power to detect an OR of 2.5 at 5% significance level in 80% or more likelihood for all lung cancer cases, squamous cell carcinoma, and nonadenocarcinoma (data not shown). For other cell types and analysis by smoking dosage, however, the statistical power was found to be lower (data not shown). The required numbers of cases, based on 80% power and 5% type I error, were 387, 80, 545, and 172 cases for total lung cancer, squamous cell carcinoma, adenocarcinoma, and nonadenocarcinoma, respectively, when the same number of cases and controls are recruited and the risk of Cys/Cys genotype versus other genotypes combined is evaluated.

Distribution in Different Populations.
As shown in Table 4 all the distributions of this genotype in Japanese (Tokyo), Chinese (Jiangsu), Hungarian Caucasians, and Micronesians were consistent with Hardy-Weinberg equilibrium, but those of Melanesians (P = 2.8 x 10^-5) and Australian Caucasians (P = 0.002) were not. The Ser/Ser genotype was less frequent in Chinese, whereas the prevalence of the Cys/Cys genotype was relatively low in Australian Caucasians, Melanesians, and Hungarians. A significant difference in genotype distributions was detected in these populations (P = 6.4 x 10^-31).

	Discussion

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We took advantage of a lung cancer case-control DNA set in Okinawa, which previously disclosed the CypIA1 Ile-Val polymorphism as a possible high-risk marker for lung cancer (13) . There are several pieces of evidence implicating oxyradical and subsequent 8-hydroxyguanine formation in human lung carcinogenesis (9 , 28 , 29) . Therefore, the interindividual differences to repair these lesions may be associated with lung cancer susceptibility. Because the content of 8-hydroxyguanine is increased in smokers’ leukocytes (26) , this association is expected to be most noticeable with tobacco-related subtypes. In a bacterial complementation assay system, the Ser allele was 7 times more capable of complementing a repair-deficient strain than the Cys allele (11) . Thus, we assigned the Ser/Ser genotype or Ser/Ser and Ser/Cys genotypes combined as reference groups (10) , but we should be aware that there is no solid biological rationale for this assignment of Ser/Ser plus Ser/Cys as a reference at this moment.

Compared to the Ser/Ser genotype, the Ser-Cys polymorphism was not associated with an increased risk of lung cancer in any subtype (Table 2) . However, when homozygous Cys/Cys were compared against the other genotypes combined, the increased risk was observed in squamous cell carcinoma and nonadenocarcinoma after adjustment for age and smoking habits (Table 3) . We still do not have a convincing explanation for our finding that only Cys/Cys was a high-risk genotype. These observations can be interpreted in several ways. Although we do not yet have in vitro data, these results are consistent with a recessive, reduced repair activity of the Cys allele. The hypothesis that only homozygous status of Cys (alternatively, the Ser allele is dominant) influences individual repair activity would explain the observation. Considering the assumed strong relationship of oxyradicals in tobacco smoke to squamous cell carcinoma, our observation that the significant association was limited to squamous cell carcinoma may reflect hOGG1 commitment in at least some of tobacco-related carcinogenesis. Consistent with this interpretation, the ORs for heavy smokers were higher (although not statistically significant) for all comparisons (Tables 2 and 3) . Currently, other subtypes such as adenocarcinoma of the lung are also considered to be associated with smoking, although the relative risk was much lower than in squamous cell and small cell carcinoma (30) . To the best of our knowledge, the involvement of oxyradicals in carcinogenesis of different subtype of lung cancer has never been addressed, and this issue remains to be investigated.

Our data may imply that the association of this genotype and lung cancer should not, however, be overemphasized. The lack of association with small cell carcinoma may be due to the low statistical power concomitant with the small number of these cancers. Our power calculation showed that our study seems to have a sufficient power to detect the risk only in squamous cell and nonadenocarcinoma. hOGG1 polymorphisms other than Ser326Cys may play a role, or the contribution of this gene to repair 8-hydroxyguanine may be small relative to other repair systems, such as the nucleotide excision repair system (31) .

As far as we know, this is the first case-control study of hOGG1 Ser326Cys polymorphism as it relates to human lung cancer, and the findings may be a local phenomenon restricted to Okinawan lung cancer. Further studies will be necessary to clarify the involvement of this gene in human lung carcinogenesis in general. It is possible that the Ser326Cys polymorphism may be involved greater to oxidative damage repair in the other organs, and thus, to carcinogenesis or other pathological conditions, such as carcinoma of the stomach (32 , 33) .

It is well known that candidate genetic markers responsible for cancer susceptibility sometimes vary in frequencies among populations. Thus, it is very important to know the basic prevalence in multiple populations to evaluate the significance of the genetic markers in cancer risk assessment (34) . The distribution of the Ser326Cys polymorphism was significantly different among several ethnic groups. Further research on the importance of hOGG1 gene in oxidative damage repair is needed to determine the applicability of the Ser326Cys polymorphism as a marker for increased cancer risk in light of the highly variable allele frequencies we have observed among populations. The differences of this polymorphism among populations will influence the interpretations and strategies for its use as a potential tool for estimation of a particular individual risk, as has been done for CYPIA1 (34, 35, 36, 37) . In the populations that have low Cys allele frequencies, like the Hungarians, our power analysis indicated that required sample sizes needed to obtain significant results would be so large that such studies would not be economically feasible. Previously, we reported an association of CYPIA1 Ile-Val polymorphism with lung cancer in this area and observed a significantly increased risk of Val/Val genotype for some of lung cancer (13) . As far as the ORs are comparable (they would be comparable if the sample size was sufficiently large and CIs of the ORs were not so wide), the lung cancer risk associated with the Ser326Cys polymorphisms appears to be smaller than that associated with the Ile-Val polymorphism of CYPIA1 (13) .

In conclusion, the Ser-Cys hOGG1 polymorphisms, especially the Cys/Cys genotype, has a potential role as a high risk marker for at least a subset of tobacco-related lung cancer. We found increased ORs among Cys/Cys individuals with higher smoking dosage, consistent with the hypothesis that smoking induced 8-hydroxyguanine is repaired less in heavy smokers with this susceptible genotype, although the association was not statistically significant.

	Footnotes

 
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported in part by a Grant-in-Aid from the Ministry of Health and Welfare (Grant 9-2); the Ministry of Health and Welfare for the Second-Term Comprehensive 10-Year Strategy for Cancer Control; the Ministry of Education, Science, Sports and Culture; the Smoking Research Foundation; and the Uehara Memorial Foundation.

² To whom requests for reprints should be addressed, at Department of Pathology, Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu 431-3192, Japan. Phone 81-53-435-2220; Fax: 81-53-435-2225; E-mail: hsugimur{at}hama-med.ac.jp

³ The abbreviations used are: SSCP, single-strand conformation polymorphism; OR, odds ratio; CI, confidence interval.

Received 2/15/99; revised 6/ 2/99; accepted 6/ 7/99.

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				L. Luna, V. Rolseth, G. A. Hildrestrand, M. Otterlei, F. Dantzer, M. Bjoras, and E. Seeberg Dynamic relocalization of hOGG1 during the cell cycle is disrupted in cells harbouring the hOGG1-Cys326 polymorphic variant Nucleic Acids Res., March 30, 2005; 33(6): 1813 - 1824. [Abstract] [Full Text] [PDF]

				E. Speina, K. D. Arczewska, D. Gackowski, M. Zielinska, A. Siomek, J. Kowalewski, R. Olinski, B. Tudek, and J. T. Kusmierek Contribution of hMTH1 to the Maintenance of 8-Oxoguanine Levels in Lung DNA of Non-Small-Cell Lung Cancer Patients J Natl Cancer Inst, March 2, 2005; 97(5): 384 - 395. [Abstract] [Full Text] [PDF]

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				L. Hagmar, U. Stromberg, S. Bonassi, I.-L. Hansteen, L. E. Knudsen, C. Lindholm, and H. Norppa Impact of Types of Lymphocyte Chromosomal Aberrations on Human Cancer Risk: Results from Nordic and Italian Cohorts Cancer Res., March 15, 2004; 64(6): 2258 - 2263. [Abstract] [Full Text] [PDF]

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				T. Arai, V. P. Kelly, K. Komoro, O. Minowa, T. Noda, and S. Nishimura Cell Proliferation in Liver of Mmh/Ogg1-deficient Mice Enhances Mutation Frequency because of the Presence of 8-Hydroxyguanine in DNA Cancer Res., July 15, 2003; 63(14): 4287 - 4292. [Abstract] [Full Text] [PDF]

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