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National Institute of Occupational Health, DK-2100 Copenhagen, Denmark [M. D., U. V., H. W., B. A. N.], and Institute of Preventive Medicine, Kommunehospitalet, DK-1399 Copenhagen, Denmark [G. F.]
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Abstract |
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Introduction |
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The XPD gene encodes a helicase involved in the nucleotide excision repair pathway (4) . In addition to repair, the XPD gene also has a function in basal transcription. Because XPD has been found to be a subunit of the transcription factor IIH required for all transcription by the RNA polymerase II, it is an essential gene (5) . Consistent with this, it has recently been reported that inactivation of the XPD gene in mice leads to embryonic lethality in the preimplantation stage (6) .
In a previous report, five different two-allele polymorphisms were found in the coding sequence of the XPD gene (7) ; and in a more recent study, several two-allele polymorphisms in five different DNA repair genes were reported (8) . The variant alleles existed at frequencies ranging from 0.04 to 0.45 in a group of 12 healthy individuals. These DNA repair gene variations remain to be studied in cohorts of cancer cases and their controls in which the finding of a higher incidence of a certain allele in cancer patients than in healthy individuals could suggest that this allele is a contributing factor in an individual’s risk of cancer.
Psoriasis patients are via their treatment exposed to a variety of genotoxic agents, including coal tar, psoralen, and methotrexate. Presumably as a consequence of the treatment psoriasis patients are at increased risk of getting BCC (9) . The risk is particularly increased among young psoriasis patients (relative risk 12; age group, 30–39 years). Young psoriasis patients, therefore, offer an attractive study group for an attempt to elucidate the role of protective mechanisms, i.e., DNA repair, in relation to cancer.
In the present study, we analyzed two known XPD polymorphisms: one silent nucleotide substitution and one amino acid substitution (7 , 8) in relation to BCC in psoriasis patients and apparently normal individuals (3) . Because both polymorphisms alter a restriction site, we developed two PCR/RFLP based assays. We performed genotyping of psoriasis patients with and without BCC and nonpsoriatic persons with and without BCC (4 x 20 persons). For both polymorphisms, we found that the distribution of the genotypes differed between cases and controls, and that the genotype was associated with the age at which the skin cancer patients had their first tumor and possibly the risk of BCC.
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Materials and Methods |
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. Group 1 included persons with a diagnosis of both psoriasis and BCC, group 2 included persons with diagnosed psoriasis, group 3 included persons with a diagnosis of BCC, and group 4 included healthy persons.
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All of the subjects gave written informed consent. The study was conducted in accordance with the Helsinki declaration and was approved by the local medical ethical committee.
DNA Extraction and PCR Analysis.
Genomic DNA was extracted from 3–5 x 106 granulocytes obtained from a blood specimen. The DNA was extracted with the Puregene DNA Isolation Kit (Gentra Systems, Minneapolis, MN).
The PCR primers were synthesized by TAG-Copenhagen Aps (Copenhagen, Denmark). The polymorphic site in exon 6 was amplified using forward primer 5'-TGG AGT GCT ATG GCA CGA TCT CT-3' and reverse primer 5'-CCA TGG GCA TCA AAT TCC TGG GA-3'. The polymorphic site in exon 23 was amplified using forward primer 5'-ATC CTG TCC CTA CTG GCC ATT C-3' and reverse primer 5'-TGT GGA CGT GAC AGT GAG AAA T-3'. The PCR reactions were initially optimized using the PCR Optimization Kit (Boehringer-Mannheim, Mannheim, Germany).
The PCR reactions were performed in a 25-µl reaction volume containing: 20 mM Tris-HCl, 50 mM KCl (pH 8.4), 1.0 mM MgCl2, 0.2 mM each deoxynucleotide triphosphate, 1.0 µM each primer, 0.5 units of Taq DNA polymerase (Life Technologies, Denmark), and 50–200 ng of genomic DNA. The cycling conditions were: initial denaturation at 96°C for 1 min, 30 cycles of denaturation at 94°C for 30 s, primer annealing at 60°C for 30 s, primer extension at 72°C for 1 min, and finally an extension at 72°C for 2 min.
Restriction Enzyme Analysis.
For both polymorphisms, only two alternative nucleotides (A and C) have been identified by DNA sequencing (7
, 8)
; therefore, in the following, we will only consider A and C as possible allele types.
Exon 6.
PCR product (10 µl) was digested with 3 units of TfiI enzyme (New England Biolabs, Beverly, MA) in a 20-µl reaction mixture, as suggested by the manufacturer, for 1.5 h and separated on a 2.0% agarose gel. The A but not the C allele in exon 6 has a TfiI restriction site within the 652-bp amplified PCR product. In addition, there is a second TfiI restriction site within the amplified fragment that serves as an internal control for digestion. The three possible genotypes are defined by three distinct banding patterns: CC (56- and 596-bp fragments), CA (56-, 114-, 482-, and 596-bp fragments), and AA (56-, 114-, and 482-bp fragments).
Exon 23.
PCR product (5 µl) was digested with 15 units of PstI enzyme (Life Technologies) in a 20-µl reaction mixture as suggested by the manufacturer for 1 h and separated on a 2.0% agarose gel. The A but not the C allele in exon 23 has a PstI restriction site within the 324-bp amplification product. In addition, there is a second PstI restriction site within the amplified fragment that serves as an internal control for digestion. The three possible genotypes are defined by three distinct banding patterns: AA (100- and 224-bp fragments), AC (66-, 100-, 158-, and 224-bp fragments), and CC (66-, 100-, and 158-bp fragments).
Host Cell Reactivation Assay.
The assay was performed basically as described by Athas et al.(10)
. Briefly, the assay measures the ability of host lymphocytes to repair a UV-damaged reporter gene inserted into a plasmid DNA that is transfected into the lymphocytes.
Statistical Methods.
The ORs and 95% CIs were calculated to assess the relationship between each polymorphism and BCC. The 
2 test was used to compare the distribution of the genotypes between BCC cases and controls. A one-sided heteroscedastic t test was used to compare the ages of first BCC between the genotypes. For both polymorphisms, the mean age of having two A alleles (AA) was compared with the mean age of having one C allele (AC), and to the mean age of having one or two C alleles (AC and CC).
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Results |
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summarizes the distribution of age and sex for the study persons. We analyzed two polymorphisms: one amino acid substitution and one silent nucleotide substitution; the distribution of the genotypes in the study groups is shown in Table 2
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C polymorphism in exon 23 at nucleotide position 35931 gives rise to the amino acid substitution LysGln. In our study population, the variant allele C had an average frequency of 0.32, which agrees with a previous study (7)
. The distribution of genotype by case-control status was comparable between psoriasis and nonpsoriasis patients (Table 2)
. Among all of the BCC cases, 95% carried at least one A allele, and 53% were homozygotes (genotype AA). Among all of the controls, 83% carried at least one A allele, and 43% were AA. The association between exon 23 genotype and risk of BCC is shown in Table 3
, and all ORs are calculated relative to subjects with the CC genotype. Subjects with the AA or AC genotypes were at higher risk of skin cancer; the ORs were 4.3 (95% CI, 0.79–23.57) and 3.7 (95% CI, 0.67–20.64), respectively.
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. The mean ages at first tumor for the genotypes AA, AC, and CC were 39, 43, and 42 years, respectively, indicating that subjects with two A alleles may have a higher risk of early BCC than subjects with one or two C alleles. The mean age at first tumor for cases with the AA genotype was significantly lower than the mean age for the AC and CC genotypes combined (P = 0.012). Comparing the mean ages for the AA and AC genotypes gave a similar result. The small number of cases with the CC genotype (n = 2) limits the ability to compare the AA or AC genotype with the CC genotype.
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C polymorphism in exon 6 at nucleotide position 22541 does not result in an amino acid change. The average frequency of the variant allele A in exon 6 was 0.48 in this study population, which is in agreement with a previous report (7)
. Among only the psoriasis patients did we observe a difference in the distribution of genotypes between the cases and controls (Table 2)
. Among psoriasis patients with BCC, 85% carried at least one A allele and 30% were homozygotes (AA). Among psoriasis patients without BCC, 60% carried at least one A allele and only 15% were AA. The association between exon 6 genotype and risk of BCC is shown in Table 3
, and the ORs are calculated relative to subjects with the CC genotype. Subjects with the AA or AC genotype were at higher risk of skin cancer; the ORs were 5.3 (95% CI, 0.78–36.31) and 3.3 (95% CI, 0.66–16.03), respectively.
The association between the genotype of exon 6 and the age at onset of skin cancer was seen only in psoriasis patients and is presented in Fig. 2
. For the psoriasis patients, the mean ages at first tumor for the genotypes AA, AC, and CC were 37, 43, and 46 years, respectively, indicating that subjects with two A alleles may have a higher risk of early BCC than subjects with one or two C alleles. The mean age at first cancer for cases with the AA genotype was marginally lower than the mean age for cases with the genotypes AC and CC combined (P = 0.060). Comparing the mean ages for the AA and AC genotypes gave a similar result. The small number of cases with the CC genotype (n = 3) precludes statistical significance when comparing the AA or AC genotype with the CC genotype. For the nonpsoriatics, the mean ages at first tumor for the genotypes AA, AC, and CC were 42, 39, and 41 years, respectively.
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. Interestingly, a person being homozygous CC in exon 23 (11%) was always homozygous CC in exon 6, and a person being homozygous AA in exon 6 (24%) was always homozygous AA in exon 23. There was a large proportion of individuals being heterozygous for the nucleotide variations in both exon 6 and exon 23 (28%). These data indicate strong linkage disequilibrium in the material and would be consistent with an absence of the exon6Aexon23C haplotype.
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Discussion |
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The frequencies of the A and C alleles in exon 23 were 0.68 and 0.32, respectively, in our study population. We found that the A allele was associated with an increased risk of BCC. Forty-eight percent of our population was homozygous AA, and they had a more than 4-fold higher risk of BCC than individuals being homozygous CC (11%). Also the heterozygotes (genotype AC) had a higher risk of BCC that was almost 4-fold increased. In addition, we found that BCC cases with the AA genotype developed their first tumors at an earlier age than cases with the AC or CC genotype. The data suggest that the C allele has a protective influence against the cancer.
The AC variation in exon 23 gives rise to the amino acid substitution LysGln, which is a change from a basic to a polar amino acid. The nucleotide variation is located about 50 bases upstream from the poly(A) signal, and could possibly improve the function of the XPD protein.
The nucleotide substitution in exon 6 appears to be very common in the population, inasmuch as the frequency of the least common allele A in our study was 0.48. Only among the psoriasis patients did we find an association of the genotype with the risk of BCC. Twenty-three percent of the psoriasis patients had the genotype AA in exon 6, and they had a more than 5-fold higher risk of BCC than the psoriasis patients with the CC genotype (28%). The heterozygotes (genotype AC) had a more than 3-fold higher BCC risk. We also observed that the psoriasis patients with BCC who carryed at least one A allele developed their first skin tumor earlier in life than the psoriasis patients with BCC carrying two C alleles.
The nucleotide substitution in exon 6 does not change an amino acid. It could conceivably affect the stability of the mRNA or disturb protein synthesis by converting a high-usage codon to a low-usage codon in the 5' proximal region of the gene (12, 13, 14) . It is, however, important to emphasize that the XPD polymorphisms studied need not be directly responsible for the differences in cancer risk. Another possible explanation is that the polymorphisms may cosegregate with another difference in XPD, whose function (or lack of function) contributes to the development of malignancy. Finally, ERCC1 is located close to XPD on chromosome region 19q13.2–13.3, along with DNA ligase and XRCC1(15 , 16) , and these genes may also cosegregate with the polymorphisms. All of the four genes are important elements in repairing DNA damage.
We do not understand why the exon 6 polymorphism had an effect only in the psoriasis groups. It is possible, that the nucleotide variation in exon 6 has no detectable consequences under ordinary circumstances but only becomes important under excessive genotoxic stress. Psoriasis patients are via their treatment exposed to a variety of genotoxic agents including coal tar, psoralen, and methotrexate, and eventually the DNA damage induced by these treatments may exceed the capacity of individuals who are homozygous for the variant A allele in exon 6.
Interpretation of our data is limited by the lack of knowledge about the functional significance of the polymorphisms on the XPD gene. None of the nucleotide variations in XPD are located in any known or hypothesized helicase/ATPase domains (17) , which might be expected given that inactivation of these domains causes loss of function as well as disease or preimplantation lethality (6 , 18) . The XPD protein is a subunit of transcription factor TFIIH, which contains at least nine subunits. It is possible, that variations in the XPD subunit may cause minor structural changes that could modulate its interactions with other subunits, thereby modifying the overall transcriptional activity of the complex.
This preliminary study raises the possibility of an association of BCC development with two polymorphisms in the XPD gene, which makes this gene an important candidate for studies in susceptibility to commonly occurring forms of cancer. However, additional studies with larger sample sizes are required to detect the small effects observed. Future case-control studies of these two and other sequence variants identified in the XPD gene (8) and characterization of the functional significance of these variants will help to an understanding of the role of the XPD gene in cancer etiology.
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Acknowledgments |
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Footnotes |
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1 Supported by the Danish Research Academy, the Danish Medical Research Council Grant 9600259, Hofbuntmager Aage Bang’s Foundation, and the Psoriasis Research Foundation. Gerda Frentz’s professorship is granted by Nycomed. 
2 The contribution of the dermatologists in private practice all over Denmark, the Departments of Dermatology of the five Danish university hospitals, and the collaborating dermato-pathologists is gratefully acknowledged. A specified list of these participants is available on request, addressed to G. Frentz, Institute of Preventive Medicine, Kommunehospitalet, Ø. Farimagsgade 5, DK-1399 Copenhagen, Denmark.
3 To whom requests for reprints should be addressed, at National Institute of Occupational Health, Lerso Parkallé 105, DK-2100 Copenhagen, Denmark. Phone: 45-3916-5200; Fax: 45-3916-5201; E-mail: ban{at}ami.dk
4 The abbreviations used are: BCC, basal cell carcinoma; OR, odds ratio; CI, confidence interval.
5 M. Dybdahl et al.Low DNA repair is a risk factor in skin carcinogenesis: a study of basal cell carcinoma in psoriasis patients, submitted for publication, 1998.
Received 7/31/98; revised 9/28/98; accepted 10/16/98.
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