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DNA Repair Polymorphisms and Risk of Colorectal Adenomatous or Hyperplastic Polyps

Cancer Prevention Program, Fred Hutchinson Cancer Research Center, Seattle, Washington

Requests for reprints: Jeannette Bigler, Cancer Prevention Program, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, M5-A864, Seattle, WA 98109. Phone: 206-667-5077; Fax: 206-667-2537. E-mail: jbigler{at}fhcrc.org

	Abstract

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Genetic variability in DNA repair genes may contribute to differences in DNA repair capacity and susceptibility to cancer, especially in the presence of exposures such as smoking. In a Minnesota-based case-control study of cases with only adenomatous polyps (n = 384), only hyperplastic polyps (n = 191), or both types of polyps (n = 119) versus polyp-free controls (n = 601), we investigated the role of polymorphisms in the DNA repair genes O⁶-methylguanine methyltransferase (MGMT; p.L84F and p.I143V), XPD (p.D312N and p.K751Q), and XPG (p.D1104H). MGMT polymorphisms were not associated with polyp risk. Overall, a homozygous variant XPD–combined genotype was associated with an increased risk of adenomatous polyps [odds ratio (OR), 1.57; 95% confidence interval (95% CI), 1.04-2.38] and an XPGHH1104 genotype with a decreased risk of hyperplastic polyps (OR, 0.36; 95% CI, 0.13-0.98). However, age stratification showed that the XPD association was present only in subjects 60 years old (OR, 3.77; 95% CI, 1.94-7.35), whereas the XPG association was observed largely in subjects <60 years old (OR, 0.20; 95% CI, 0.05-0.91). Smokers did not have a significantly increased risk of adenomatous polyps in the absence of synchronous hyperplastic polyps, except for subjects with a homozygous variant XPD genotype or a homozygous wild-type XPG genotype (OR, 3.93; 95% CI, 1.68-9.21 and OR, 1.59; 95% CI, 1.01-2.50, respectively). Smoking was associated with a statistically significant 2.5- to 6-fold increased risk of hyperplastic polyps for individuals with most of the DNA repair genotypes. However, no substantial increase was observed among individuals who were homozygous variant for XPG (1104HH; OR, 1.38; 95% CI, 0.25-7.65). Our data suggest that polymorphisms in DNA repair genes may be risk factors for colorectal neoplasia and that they may exacerbate the effects of exposures to carcinogens.

	Introduction

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Carcinogens can interact with DNA to form bulky adducts that can lead to incorrect base pairing in the next replication cycle and eventually to cancer. Possible sources of carcinogens include dietary, lifestyle, and occupational exposures (1); endogenous alkylating agents; and in situ carcinogen formation mediated by the bacterial or chemical nitrosation of amines (2). However, an extensive system of DNA repair enzymes can reverse damage to DNA. Nucleotide excision repair (NER) is a major pathway that removes bulky DNA adducts and helix-distorting lesions. Included among the NER genes are the Xeroderma pigmentosum (XP) genes. XPD (also known as ERCC2) encodes an ATP-dependent helicase that is a component of the transcription factor complex TFIIH, which participates in both NER and basal transcription (3). XPD plays an important role in DNA repair by eliminating bulky DNA adducts produced by environmental toxins and xenobiotics through the NER pathway (3). XPG (also known as ERCC5) is a structure-specific endonuclease that cleaves damaged DNA approximately five nucleotides 3' to the site of the lesion and is also required nonenzymatically for subsequent 5' incision by the XPF/ERCC1 heterodimer during the NER process (4-6).

O⁶-methylguanine methyltransferase (MGMT) is a ubiquitous repair protein that plays a vital role in minimizing the mutagenic effects of alkylating agents. Unlike the NER genes, which excise damaged DNA, MGMT is able to act as a single protein that reverses alkylation damage (7). It catalyzes the transfer of a variety of alkyl groups from guanine to an active site cysteine. MGMT seems to lack the ability to dealkylate itself and participates in a single reaction and is thereby irreversibly inactivated (8, 9). Point mutations induced by alkylating agents originate principally from O⁶-alkylguanine and O⁴-alkylthymine (10). Alkylated adenine derivatives have been found in the urine of smokers (11) and procarcinogenic DNA adducts that arise from exposure to methylating agents have been detected in human colorectal DNA at levels comparable with those that cause adverse effects in model systems (2).

Genetic alterations in the DNA repair genes can impair their function and lead to an altered neoplasia risk. Defective NER is associated with rare, autosomal recessive inherited disorders (12). XP patients are extremely susceptible to sunlight and have a high incidence of skin cancers (12) as well as an increased risk of internal cancers (13). However, several amino acid changes in NER genes occur at polymorphic frequencies in healthy individuals (14-16). Two polymorphic amino acid changes were identified in XPD, p.D312N, and p.K751Q, which have allele frequencies between 0.35 and 0.44 (for review, see ref. 17). The functional effect of the XPD polymorphisms is unclear. Whereas some researchers have reported impaired repair of UV-induced DNA damage or of aromatic DNA adducts in the presence of XPD variant alleles (18-24), others found no association of XPD polymorphisms and the capacity to repair benzo(a)pyrene diol epoxide DNA adducts or chromatid aberrations (25-28). The cause of these apparently contradictory results may be the different assay systems used and the different types of DNA damage investigated.

The XPG p.D1104H polymorphism has an allele frequency of 0.25 in Caucasians (14, 29) and 0.5 in Koreans (30). XPG p.D1104H homozygous variant individuals seemed to have a somewhat higher thymidine-dimer repair rate than homozygous wild-type individuals, whereas no difference was observed in the repair of thymidine-cytidine dimers (29).

Three polymorphisms leading to amino acid changes have been identified in MGMT (p.L84F, p.I143V, and p.K178R; refs. 31-33). The p.I143V and the K178R polymorphisms seem to be in linkage disequilibrium (32, 34). The frequencies of MGMT variant alleles in Caucasians are around 0.12 (32-35). Because the p.I143V polymorphism is close to the alkyl acceptor Cys¹⁴⁵ of the MGMT active site and this amino acid is conserved among mammalian MGMTs, this mutation may affect the function of MGMT.

The association of XPD polymorphisms and risk of neoplasia has been explored for a number of cancers (for review, see ref. 17). The data on DNA repair gene polymorphisms are not consistent by specific gene or by cancer site—at least in part because of underpowered studies. However, given different exposures and doses for different cancers, heterogeneity is expected (23, 29, 30, 34-50).

Adenomatous and hyperplastic colorectal polyps have several similar risk factors. Male sex, smoking, and alcohol consumption have been associated with an increased risk; on the other hand, nonsteroidal anti-inflammatory drugs (NSAID) use, postmenopausal hormone therapy, and calcium intake tend to be associated with a reduced risk (51). However, age seems to be a risk factor for adenomatous but not hyperplastic polyps. Smoking, on the other hand, is associated with a higher risk of hyperplastic than of adenomatous polyps (51). In the present study, we investigated whether altered DNA repair capacity due to genetic polymorphisms influenced the risk of adenomatous or hyperplastic polyps in the presence of a number of environmental exposures.

	Materials and Methods

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Study Participants
This case-control study was conducted between April 1991 and April 1994 as part of the Minnesota Cancer Prevention Research Unit, a National Cancer Institute–funded program project that combined several units within the University of Minnesota and Digestive Healthcare, a large multiclinic private gastroenterology practice. Participant recruitment for this case-control study has been described previously (52). Briefly, patients ages 30 to 74 years who were scheduled for a colonoscopy were recruited before colonoscopy so as to blind patients and recruiters to the final diagnosis. Patients with colorectal adenomatous and/or hyperplastic polyps and polyp-free controls without individual history of ulcerative colitis, Crohn's disease, adenomatous polyps, and cancer (except nonmelanoma skin cancer), and who were free of known genetic syndromes associated with a predisposition to colonic neoplasia, were recruited into the study. The study was approved by the internal review boards of the University of Minnesota and each endoscopy site. Written informed consent was obtained.

Based on colonoscopic and pathologic findings, participants were assigned to groups of colorectal adenomatous polyps only (n = 395), hyperplastic polyps only (n = 196), or both types of polyps (n = 122). Controls (n = 621) were polyp-free at colonoscopy. Patients for whom the colonoscopy did not reach the cecum were ineligible; removed polyps were examined histologically using standard diagnostic criteria (53).

Information on use of aspirin and NSAID, lifestyle factors and diet, anthropometry, demographics, and medical information, including family history of cancer and polyps, was obtained by questionnaire. The participation rate for all colonoscoped patients was 68%.

The activities associated with the present study were approved by the Institutional Review Board of the Fred Hutchinson Cancer Research Center.

Genotyping
Genomic DNA was extracted from peripheral WBC using the Puregene kit (Gentra Systems, Minneapolis, MN). DNA was available for genotyping for 601 controls, 384 adenoma cases, 191 hyperplastic polyp cases, and 119 cases with both adenomatous and hyperplastic polyps. Missing genotyping data were due to lack of amplification or ambiguous results (<0.5 %). Polymorphisms in XPD (p.D312N, p.K751Q), MGMT (p.L84F, p.I143V), and XPG (p.D1104H) were detected by allelic discrimination using a 5' nuclease assay on a 7900HT sequence detection system (Applied Biosystems, Foster City, CA). The 5' nuclease genotyping assays were validated by genotyping 100 individuals by both 5' nuclease assay and RFLP or sequencing. There were no discrepancies between assays. Primers and minor-groove-binding probes with nonfluorescent quenchers were obtained from Applied Biosystems. Genotyping assays were done in 20 µL reactions containing 200 nmol/L of each amplification primer, 100 nmol/L of each probe, 1x Taqman core reagents (Applied Biosystems) or universal master mix (Applied Biosystems), MgCl₂, and 4 ng genomic DNA. The 40 amplification cycles were preceded by incubations at 50°C for 2 minutes and denaturation at 94°C for 10 minutes. Primer and probe sequences, MgCl₂ concentrations, and amplification variables for each polymorphism are listed in Table 1. Positive controls for all the genotypes as well as four negative controls were included in each plate. For quality control purposes, genotyping for 94 randomly selected samples was repeated. There were no discrepancies. All genotypes were in Hardy-Weinberg equilibrium.

	Results

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DNA Repair Polymorphisms
Characteristics of the study population and risk factors for colorectal polyps have been described previously (51, 52, 55, 56). Briefly, adenoma cases were older than individuals with hyperplastic polyps or polyp-free controls, and are more likely to be male.

We genotyped the study participants for two polymorphisms in MGMT (p.L84F and p.I143V) and XPD (p.D312N and p.K751Q) and the p.D1104H polymorphism in XPG. Allele frequencies of 0.12 and 0.12 for MGMT p.L84F and p.I143V, 0.37 and 0.39 for XPDp.D312N and p.K751Q, and 0.23 for XPGp.D1104H are comparable with those for Caucasians in other studies (14, 25, 29, 33, 35, 57-59). There was partial linkage disequilibrium between the two polymorphisms in MGMT, with the haplotype carrying both variants being the least frequent haplotype (see Table 2). Partial linkage disequilibrium was also present between the polymorphisms in XPD, where the haplotype with both variants was the most frequent non-wild-type haplotype.

Main Associations of DNA Repair Polymorphisms
Because of their different risk profiles, cases were stratified by diagnosis (i.e., whether they were diagnosed with only adenomatous polyps, only hyperplastic polyps, or both types of polyps). Table 3 shows the main associations of individual genotypes for each gene. No association was observed between MGMT genotypes and risk of any phenotype. There was a statistically significantly increased adenoma risk for individuals with two XPD variant alleles. XPG homozygous variant individuals had a significantly decreased risk of developing hyperplastic polyps.

Effect of DNA Repair Polymorphisms as a Factor of Age
In this study population, we showed earlier that age was a strong risk factor for developing adenomatous polyps only or both types of polyps, but not hyperplastic polyps (51). Women were at lower risk of hyperplastic as well as adenomatous polyps. We, therefore, tested whether DNA repair polymorphisms affected these relationships.

In keeping with earlier analyses of this study population (51), we dichotomized the age groups at 60 years of age, which represents an approximate median split of the age distribution. None of the polymorphisms modified the increased risk of adenomatous polyps in the older subjects (Table 4). However, the inverse association between hyperplastic polyp risk and a homozygous variant XPG genotype was observed only in the <60-year-old participant group (OR, 0.20; 95% CI, 0.05-0.91, P_interaction = 0.07). In contrast, a homozygous variant XPD combined genotype was associated with an increased risk of the hyperplastic polyps phenotype in the 60-year-old participants (OR, 2.54; 95% CI, 1.10-5.83). Among XPG homozygous variant individuals, the risk of developing both types of polyps was higher in the 60-year age group than in the <60-year age group (OR, 8.38; 95% CI, 2.33-30.13 versus OR, 0.25; 95% CI, 0.03-1.98; see Table 4). Among XPG homozygous wild-type individuals, those age 60 years had an 3-fold higher risk of both types of polyps than study subjects who were <60 years old. However, these risk ratios were based on small numbers and, thus, the P value testing for gene-age interaction was not statistically significant. There were no significant gene-age interactions for adenomatous polyps only.

Other Risk Factors
Other exposures that can alter risk of polyps or can potentially affect DNA repair include alcohol and vitamin intake (folate, vitamin B₆, and vitamin B₁₂), as well as meat consumption. None of these exposures, in conjunction with DNA repair polymorphisms, resulted in a significantly increased or decreased risk for any of the polyps (data not shown). Furthermore, there were no sex-specific differences in the associations of DNA repair polymorphisms and polyp risk. Because polymorphisms in individual DNA repair genes were risk factors for colorectal polyps, we examined whether an increasing number of variant DNA repair alleles had an additive effect. The observed main associations for XPD and adenomatous polyps and XPG and hyperplastic polyps persisted but were not modified by the presence of polymorphisms in another gene (data not shown).

	Discussion

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DNA repair genes have been studied in the context of risk factors for developing cancer and as predictors of response of patients to chemotherapy and survival. Associations between XPD, XPG, and MGMT polymorphisms and colorectal neoplasia have not been explored extensively. The only study exploring colorectal cancer risk and NER polymorphisms found no associations (42). In our study population, XPD homozygous variant individuals were at increased risk of adenomatous polyps. The difference between the two studies may in part be due to the study size and the type of analysis done. The study of Mort et al. (42) was small (45 cases and 44-71 controls) and allele frequencies in cases and controls were used to calculate ORs. Unlike the analyses presented here (based on genotype frequencies), this approach reduces the sensitivity of the analyses.

An increased risk of colorectal adenomas among XPD homozygous variants is consistent with the decreased DNA repair capacity of variant enzymes, which has been observed in some (18-24), but not all (25, 27, 28), functional studies. Heavy smokers have a higher carcinogen exposure and are, therefore, more likely to have a higher level of DNA adducts (60-62). The approximately 4-fold increased risk of an adenomatous polyp in homozygous variant XPD smokers compared with wild-type nonsmokers also suggests impaired DNA repair capacity in these individuals.

The polymorphisms in XPD do not seem to alter gene function dramatically. However, even a slight impairment in function may eventually be detrimental, especially in the presence of steady and high exposure to DNA-damaging agents. The inverse association between XPG genotype and hyperplastic polyp risk is observed only among younger individuals, suggesting perhaps that a longer exposure to DNA-damaging agents may overcome a possible genetic advantage. It remains to be established what degree, if any, of functional improvement is associated with the XPG variant allele.

In this study, MGMT genotype was not associated with altered polyp risk. There are no studies that clearly show a functional effect of the polymorphisms. However, the p.I143V polymorphism is close to the MGMT active site and the wild-type allele is generally conserved among mammalian MGMTs, which suggests that this mutation may affect function. It is possible that in vivo, MGMT genotype effects are less obvious because the function of this gene can also be impaired by methylation. Whereas a number of studies showed that MGMT can be hypermethylated in tumors, including colorectal tumors (63), methylated alleles can be found in normal tissue as well.¹

Folate, vitamin B₆, and vitamin B₁₂ intake did not modulate the associations of DNA repair genotypes and polyp risk. Folate metabolites provide methyl groups for a number of pathways, including purine and pyrimidine synthesis, and high folate intake has been associated with decreased colorectal neoplasia risk (64, 65). The lack of association of NER polymorphisms, folate, and vitamin B₆/B₁₂ intake, and colorectal polyp risk suggests that nucleotide synthesis is not a limiting factor in the repair of DNA damage in this population.

In this study, the XPD homozygous variant individuals were at an increased risk of adenomatous polyps but XPG homozygous variant individuals were at a decreased risk of hyperplastic polyps. This observation suggests that the polymorphisms affect function in a different manner. These genotype associations persisted in the stratified analyses and further support the observation that adenomatous and hyperplastic polyps have different risk profiles as we have reported earlier (51).

The study population in this case-control study was not necessarily representative of the population, because only individuals who underwent colonoscopy were eligible. Individuals in the control group were more likely to have a family history as an indication for colonoscopy and probably underwent screening for this reason. On the other hand, the major advantage of this clinic-based approach was that the presence of polyps was clearly established and that the control group was free of any polyps. Studies that use a population-based unscreened control group include a substantial proportion of individuals with undetected polyps, which will attenuate any study findings. Furthermore, considering the large number of tests done to evaluate gene-environment interactions, results should be interpreted with caution.

Another aspect of carcinogen exposure, DNA damage, DNA repair, and colorectal neoplasia risk is the role of biotransformation enzymes, which show substantial genetic variability. The efficiency with which a carcinogen is inactivated and eliminated from the body may influence the amount of DNA damage that occurs. The interplay between genetic variants in biotransformation enzymes and in DNA repair capacity will need to be explored in future studies.

	Acknowledgments

 
We thank Dr. Roberd Bostick and Lisa Fosdick for their contributions to establishing the case-control study and Juanita Leija for performing the genotyping assays.

	Footnotes

 
Grant support: NIH grant RO1 CA059045 (J.D. Potter).

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ J. Bigler et al., unpublished data.

Received 4/19/05; revised 8/17/05; accepted 9/ 9/05.

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