This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Huang, H.-Y. Articles by Appel, L. J. Search for Related Content PubMed PubMed Citation Articles by Huang, H.-Y. Articles by Appel, L. J.

The Effects of Vitamin C and Vitamin E on Oxidative DNA Damage: Results from a Randomized Controlled Trial¹

Department of Epidemiology, School of Hygiene and Public Health [H-Y. H., K. J. H.], and Department of Medicine, School of Medicine [L. J. A.], Johns Hopkins University, Baltimore, Maryland 21205-2223

Oxidative DNA damage may be important in mutagenic, carcinogenic, and aging processes. Although it is plausible that antioxidant vitamins may reduce oxidative DNA damage, evidence from human studies has been sparse and inconsistent. We determined the short-term effects of vitamin C (500 mg/day) and vitamin E (400 IU d-- tocopheryl acetate/day) supplements on oxidative DNA damage in a double-masked, placebo-controlled, 2x2 factorial trial in 184 nonsmoking adults. Mean duration of supplementation was 2 months. Oxidative DNA damage was measured by 24-h urinary excretion of 8-hydroxy-2'-deoxyguanosine (8-OHdG). At baseline, urinary 8-OHdG (mean ± SE; ng/mg creatinine) was associated with race (15.6 ± 0.8 in African Americans versus 20.3 ± 1.2 in Caucasians, P = 0.001), prior antioxidant supplement use (18.6 ± 0.8 in users versus 13.8 ± 1.5 in non-users, P = 0.007), and regular exercise (19.2 ± 1.1 in exercisers versus 16.6 ± 0.9 in non-exercisers, P = 0.04). Fruit and vegetable intake and serum ascorbic acid were inversely associated with urinary 8-OHdG (P-trend = 0.02 and 0.016, respectively). The benefits of fruit and vegetable intake became evident with the consumption being at least three servings/day. At the end of supplementation, change from baseline in urinary 8-OHdG (mean ± SE; ng/mg creatinine) was -0.6 ± 1.4 (P = 0.61), 0.6 ± 1.1 (P = 0.59), 0.5 ± 1.0 (P = 0.61), and 1.6 ± 1.4 (P = 0.27) in the placebo, vitamin C alone, vitamin E alone, and combined vitamins C and E groups, respectively. In overall and subgroup analyses, there was no significant main effect or interaction effect of the supplements on urinary 8-OHdG. In conclusion, supplementation of diet with vitamin C (500 mg/day) and vitamin E (400 IU d--tocopheryl acetate/day) had no significant main effect or interaction effect on oxidative DNA damage as measured by urinary 8-OHdG in nonsmoking adults. However, several aspects of a healthy lifestyle were associated with lower oxidative DNA damage.

This article has been cited by other articles:

				Y. Cui, J. M Shikany, S. Liu, Y. Shagufta, and T. E Rohan Selected antioxidants and risk of hormone receptor-defined invasive breast cancers among postmenopausal women in the Women's Health Initiative Observational Study Am. J. Clinical Nutrition, April 1, 2008; 87(4): 1009 - 1018. [Abstract] [Full Text] [PDF]

				G. Borthakur, C. Butryee, M. Stacewicz-Sapuntzakis, and P. E. Bowen Exfoliated Buccal Mucosa Cells as a Source of DNA to Study Oxidative Stress Cancer Epidemiol. Biomarkers Prev., January 1, 2008; 17(1): 212 - 219. [Abstract] [Full Text] [PDF]

				J. L. Watters, J. A. Satia, L. L. Kupper, J. A. Swenberg, J. C. Schroeder, and B. R. Switzer Associations of Antioxidant Nutrients and Oxidative DNA Damage in Healthy African-American and White Adults Cancer Epidemiol. Biomarkers Prev., July 1, 2007; 16(7): 1428 - 1436. [Abstract] [Full Text] [PDF]

				S. Rohrmann, E. Giovannucci, W. C Willett, and E. A Platz Fruit and vegetable consumption, intake of micronutrients, and benign prostatic hyperplasia in US men Am. J. Clinical Nutrition, February 1, 2007; 85(2): 523 - 529. [Abstract] [Full Text] [PDF]

				L. A Boothby and P. L Doering Vitamin C and Vitamin E for Alzheimer's Disease Ann. Pharmacother., December 1, 2005; 39(12): 2073 - 2079. [Abstract] [Full Text] [PDF]

				B. Frei Efficacy of Dietary Antioxidants to Prevent Oxidative Damage and Inhibit Chronic Disease J. Nutr., November 1, 2004; 134(11): 3196S - 3198S. [Full Text] [PDF]

				K. W. Lee, H. J. Lee, Y.-J. Surh, and C. Y. Lee Vitamin C and cancer chemoprevention: reappraisal Am. J. Clinical Nutrition, December 1, 2003; 78(6): 1074 - 1078. [Abstract] [Full Text] [PDF]

				H.-Y. Huang and L. J. Appel Supplementation of Diets with {alpha}-Tocopherol Reduces Serum Concentrations of {gamma}- and {delta}-Tocopherol in Humans J. Nutr., October 1, 2003; 133(10): 3137 - 3140. [Abstract] [Full Text] [PDF]

				M. K. Kim, S. Sasazuki, S. Sasaki, S. Okubo, M. Hayashi, and S. Tsugane Effect of Five-Year Supplementation of Vitamin C on Serum Vitamin C Concentration and Consumption of Vegetables and Fruits in Middle-Aged Japanese: A Randomized Controlled Trial J. Am. Coll. Nutr., June 1, 2003; 22(3): 208 - 216. [Abstract] [Full Text]

				S. T. Mayne Antioxidant Nutrients and Chronic Disease: Use of Biomarkers of Exposure and Oxidative Stress Status in Epidemiologic Research J. Nutr., March 1, 2003; 133(3): 933S - 940. [Abstract] [Full Text] [PDF]

				S. J. Padayatty, A. Katz, Y. Wang, P. Eck, O. Kwon, J.-H. Lee, S. Chen, C. Corpe, A. Dutta, S. K Dutta, et al. Vitamin C as an Antioxidant: Evaluation of Its Role in Disease Prevention J. Am. Coll. Nutr., February 1, 2003; 22(1): 18 - 35. [Abstract] [Full Text] [PDF]

				J. S. Jacobson, M. D. Begg, L. W. Wang, Q. Wang, M. Agarwal, E. Norkus, V. N. Singh, T.-L. Young, D. Yang, and R. M. Santella Effects of a 6-Month Vitamin Intervention on DNA Damage in Heavy Smokers Cancer Epidemiol. Biomarkers Prev., December 1, 2000; 9(12): 1303 - 1311. [Abstract] [Full Text]

				B. Halliwell Why and how should we measure oxidative DNA damage in nutritional studies? How far have we come? Am. J. Clinical Nutrition, November 1, 2000; 72(5): 1082 - 1087. [Abstract] [Full Text] [PDF]