This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hu, J. J. Articles by D’Agostino, R. B. Search for Related Content PubMed PubMed Citation Articles by Hu, J. J. Articles by D’Agostino, R. B., Jr.

-Tocopherol Dietary Supplement Decreases Titers of Antibody against 5-Hydroxymethyl-2'-Deoxyuridine (HMdU)¹

Lombardi Cancer Center, Georgetown University Medical Center, Washington, DC 20007 [J. J. H., B. N. S., J. J. H.]; Department of Epidemiology and Biostatistics, Memorial Sloan-Kettering Cancer Center, New York, New York 10021 [M. B., S. M.]; Department of Environmental Medicine, New York University School of Medicine, New York, New York 10016 [C. X. C., K. F.]; and Department of Public Health Sciences, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157 [R. B. D.]

This study evaluated the effects of vitamin E (-tocopherol) on oxidative DNA damage in a randomized double-blind Phase II chemoprevention trial. Oxidative DNA damage was measured by the level of auto-antibody (Ab) against 5-hydroxymethyl-2'-deoxyuridine (HMdU) in plasma. After the baseline screening, eligible subjects (n = 31; plasma samples from 28 subjects were available for this study) were randomized to receive 15, 60, or 200 mg of -tocopherol per day for 28 days. Biomarkers were measured twice at baseline—on day 1 (visit 1) and day 3 (visit 2)—and twice after intervention—on day 17 (visit 3) and day 31 (visit 4). At baseline, there was a highly significant inverse correlation between anti-HMdU Ab titer and plasma vitamin E level (r = -0.53; P = 0.004; n = 28). Smoking did not affect baseline anti-HMdU Ab titer; however, anti-HMdU Ab titer levels at baseline were significantly lower in subjects with above-median (0.75 ounce/day) alcohol consumption (P = 0.008). No significant change in anti-HMdU Ab level occurred at either visit 3 or visit 4 for subjects on the lowest dose, 15 mg -tocopherol per day. Subjects receiving 60 mg of -tocopherol per day had a significant decrease in anti-HMdU Ab level at visits 3 and 4 compared with baseline (P = 0.049 and P = 0.02, respectively). However, subjects receiving the highest dose, 200 mg/day, had less consistent results: a significant decrease in anti-HMdU Ab level was seen at visit 4 (P = 0.04) but not at visit 3. Our results demonstrate an inverse relationship between -tocopherol and anti-HMdU Abs in plasma; oxidative DNA damage can be modulated by short-term dietary supplementation of -tocopherol in some subjects.

This article has been cited by other articles:

				I. Romieu, F. Castro-Giner, N. Kunzli, and J. Sunyer Air pollution, oxidative stress and dietary supplementation: a review Eur. Respir. J., January 1, 2008; 31(1): 179 - 197. [Abstract] [Full Text] [PDF]

				J.-Y. Han, D. D. Liu, J. J. Lee, J. Kurie, R. Lotan, W. K. Hong, and H.-Y. Lee 9-cis-Retinoic Acid Treatment Increases Serum Concentrations of {alpha}-Tocopherol in Former Smokers Clin. Cancer Res., March 15, 2005; 11(6): 2305 - 2311. [Abstract] [Full Text] [PDF]

				P. Wallstrom, K. Frenkel, E. Wirfalt, B. Gullberg, J. Karkoszka, J. Seidegard, L. Janzon, and G. Berglund Antibodies against 5-Hydroxymethyl-2'-deoxyuridine Are Associated with Lifestyle Factors and GSTM1 Genotype: A Report from the Malmo Diet and Cancer Cohort Cancer Epidemiol. Biomarkers Prev., May 1, 2003; 12(5): 444 - 451. [Abstract] [Full Text] [PDF]

				S. T. Mayne Antioxidant Nutrients and Chronic Disease: Use of Biomarkers of Exposure and Oxidative Stress Status in Epidemiologic Research J. Nutr., March 1, 2003; 133(3): 933S - 940. [Abstract] [Full Text] [PDF]

				T. Hagenlocher, J. Nair, N. Becker, A. Korfmann, and H. Bartsch Influence of Dietary Fatty Acid, Vegetable, and Vitamin Intake on Etheno-DNA Adducts in White Blood Cells of Healthy Female Volunteers: A Pilot Study Cancer Epidemiol. Biomarkers Prev., November 1, 2001; 10(11): 1187 - 1191. [Abstract] [Full Text] [PDF]

				L. A. Mooney, F. P. Perera, A. M. Van Bennekum, W. S. Blaner, J. Karkoszka, L. Covey, Y. Hsu, T. B. Cooper, and K. Frenkel Gender Differences in Autoantibodies to Oxidative DNA Base Damage in Cigarette Smokers Cancer Epidemiol. Biomarkers Prev., June 1, 2001; 10(6): 641 - 648. [Abstract] [Full Text] [PDF]