| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Cancer Epidemiology Biomarkers & Prevention, Vol 7, Issue 4 303-308, Copyright © 1998 by American Association for Cancer Research
ARTICLES |
Y Li, RC Millikan, S Carozza, B Newman, E Liu, R Davis, R Miike and M Wrensch
Department of Epidemiology, School of Public Health, University of North Carolina, Chapel Hill 27599-7400, USA.
A population-based series of incident cases of malignant glioma were analyzed for mutations in the tumor suppressor gene p53. Exons 4-8 were screened using PCR-single-strand conformation analysis and confirmed through direct sequencing. Of 62 tumors analyzed, 12 (19%) contained mutations in p53: one 18-bp duplication in exon 5, five point mutations in exon 4, three point mutations in exon 7, two point mutations in exon 8, and a splice-site mutation at the exon 6/intron 7 boundary. In contrast to previous studies of malignant glioma, the prevalence of transversion mutations (56%) was higher than transition mutations (33%). A large proportion of transversion mutations occurred in exon 4, a region that is not routinely screened in gliomas. We present here an improved method for screening exon 4 (and other GC-rich regions) of p53 using PCR-single-strand conformation analysis. The high frequency of transversion mutations suggests a role for exogenous carcinogens in the etiology of malignant glioma.
This article has been cited by other articles:
|
|
 |
|
  M. E. Scheurer, C. J. Etzel, M. Liu, R. El-Zein, G. E. Airewele, B. Malmer, K. D. Aldape, J. S. Weinberg, W.K. A. Yung, and M. L. Bondy Aggregation of Cancer in First-Degree Relatives of Patients with Glioma Cancer Epidemiol. Biomarkers Prev., November 1, 2007; 16(11): 2491 - 2495. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Wrensch, Y. Minn, T. Chew, M. Bondy, and M. S. Berger Epidemiology of primary brain tumors: Current concepts and review of the literature Neuro-oncol, October 1, 2002; 4(4): 278 - 299. [Abstract] [PDF]
|
|
|
|
 |
|
 K. Conway, S. N. Edmiston, L. Cui, S. S. Drouin, J. Pang, M. He, C.-K. Tse, J. Geradts, L. Dressler, E. T. Liu, et al. Prevalence and Spectrum of p53 Mutations Associated with Smoking in Breast Cancer Cancer Res., April 1, 2002; 62(7): 1987 - 1995. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Chakravarti, M. A. Delaney, E. Noll, P. McL. Black, J. S. Loeffler, A. Muzikansky, and N. J. Dyson Prognostic and Pathologic Significance of Quantitative Protein Expression Profiling in Human Gliomas Clin. Cancer Res., August 1, 2001; 7(8): 2387 - 2395. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
