Cancer Epidemiology Biomarkers & Prevention, Vol 2, Issue 1 59-62, Copyright © 1993 by American Association for Cancer Research

ARTICLES

Polycyclic aromatic hydrocarbon-DNA adducts in white blood cells and urinary 1-hydroxypyrene in foundry workers

RM Santella, K Hemminki, DL Tang, M Paik, R Ottman, TL Young, K Savela, L Vodickova, C Dickey and R Whyatt
Division of Environmental Science, School of Public Health, Columbia University, New York, New York 10032.

In an ongoing comprehensive evaluation of biological markers, workers in or near an iron foundry with varying exposure to polycyclic aromatic hydrocarbons (PAH) were analyzed for molecular response to this exposure. Exposure to benzo(a)pyrene, determined by personal monitors worn by the workers (2 to 60 ng/m3), was considerably lower than in a previous study at this foundry (< 50 to 200 ng/m3) (F.P. Perera et al., Cancer Res., 48: 2288-2291, 1988). Two biomarkers, 1-hydroxypyrene in urine measured by high-performance liquid chromatography with fluorescence detection (a measure of internal dose) and PAH-DNA adducts in WBC measured by immunoassay (a measure of biologically effective dose) were assessed to demonstrate their relationship to the lowest exposures yet analyzed in foundry workers. In addition, these markers were analyzed for dose response and interindividual variability. Cigarette smoking, but not age or charbroiled food, influenced the level of 1-hydroxypyrene but not PAH-DNA adducts. When workers were classified into three exposure categories (low, medium, and high), mean 1-hydroxypyrene levels were 2.7, 1.8, and 3.6 mumol/mol creatinine, respectively. Comparisons by analysis of variance showed a significant difference between the groups after controlling for smoking (P = 0.02), but a trend test using multivariate linear regression analysis was not significant (r = 0.27; P = 0.07). Substantial interindividual variation was demonstrated by the 19- to 20-fold range in the values within each of the three exposure groups.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				E. Gyorffy, L. Anna, K. Kovacs, P. Rudnai, and B. Schoket Correlation between biomarkers of human exposure to genotoxins with focus on carcinogen-DNA adducts Mutagenesis, January 1, 2008; 23(1): 1 - 18. [Abstract] [Full Text] [PDF]

				H. C. A. BRANDT and W. P. WATSON Monitoring Human Occupational and Environmental Exposures to Polycyclic Aromatic Compounds Ann. Hyg., July 1, 2003; 47(5): 349 - 378. [Abstract] [Full Text] [PDF]

				R R W Gaertner and G P Theriault Risk of bladder cancer in foundry workers: a meta-analysis Occup. Environ. Med., October 1, 2002; 59(10): 655 - 663. [Abstract] [Full Text] [PDF]

				P. Georgiadis, J. Topinka, M. Stoikidou, S. Kaila, M. Gioka, K. Katsouyanni, R. Sram, H. Autrup, and S. A. Kyrtopoulos Biomarkers of genotoxicity of air pollution (the AULIS project): bulky DNA adducts in subjects with moderate to low exposures to airborne polycyclic aromatic hydrocarbons and their relationship to environmental tobacco smoke and other parameters Carcinogenesis, September 1, 2001; 22(9): 1447 - 1457. [Abstract] [Full Text] [PDF]

				R. W.L. Godschalk, E. J.C. Moonen, P. A.E.L. Schilderman, W. M.R. Broekmans, J. C.S. Kleinjans, and F. J. Van Schooten Exposure-route-dependent DNA adduct formation by polycyclic aromatic hydrocarbons Carcinogenesis, January 1, 2000; 21(1): 87 - 92. [Abstract] [Full Text] [PDF]

				R. M. Santella Immunological Methods for Detection of Carcinogen-DNA Damage in Humans Cancer Epidemiol. Biomarkers Prev., September 1, 1999; 8(9): 733 - 739. [Full Text]

				S. Pavanello, D. Favretto, F. Brugnone, G. Mastrangelo, G. D. Pra, and E. Clonfero HPLC/fluorescence determination of anti-BPDE–DNA adducts in mononuclear white blood cells from PAH-exposed humans Carcinogenesis, March 1, 1999; 20(3): 431 - 435. [Abstract] [Full Text] [PDF]