This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Castaño-Vinyals, G. Articles by Vermeulen, R. Search for Related Content PubMed PubMed Citation Articles by Castaño-Vinyals, G. Articles by Vermeulen, R.

Bulky DNA Adduct Formation and Risk of Bladder Cancer

¹ Centre for Research in Environmental Epidemiology and ² Unitat de Biologia Cellular i Molecular, Institut Municipal d'Investigació Mèdica; ³ Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra; ⁴ CIBER Epidemiología y Salud Pública, Barcelona, Spain; ⁵ Department of Environmental Health, University of Cincinnati, Cincinnati, Ohio; ⁶ Division of Cancer Epidemiology and Genetics, National Cancer Institute, Department of Health and Human Services, Bethesda, Maryland; ⁷ Área de Medicina Preventiva, Departamento de Biología Ambiental y Salud Pública, Universidad de Huelva, Huelva, Spain; ⁸ Consorci Hospitalari Parc Taulí, Sabadell, Spain; ⁹ Hospital General de Elche, Elche, Spain; ¹⁰ Universidad de Oviedo, Oviedo, Spain; ¹¹ Unidad de Investigación, Hospital Universitario de Canarias, La Laguna, Tenerife, Spain; ¹² Department of Social Medicine, Medical School, University of Crete, Herakleion, Crete, Greece; and ¹³ Institute for Risk Assessment Sciences, Utrecht University, Utrecht, the Netherlands

Requests for reprints: Gemma Castaño-Vinyals, Centre for Research in Environmental Epidemiology, Institut Municipal d'Investigació Mèdica, Doctor Aiguader, 88, 08003 Barcelona, Spain. Phone: 34-933-160-643; Fax: 34-933-160-635. E-mail: gcastano{at}imim.es

Exposure to polycyclic aromatic hydrocarbons (PAHs) has been associated with risk of bladder cancer and with increased bulky DNA adduct levels in several studies, mainly in smokers. We investigated the relation between bulky PAH-DNA adducts in peripheral blood mononuclear cells and bladder cancer in nonsmoking subjects from a large hospital-based case-control study in Spain. Additionally, we examined the association between DNA adduct formation and several air pollution proxies. The study comprised 76 nonsmoking cases and 76 individually matched controls by sex, region of residence, age, and smoking status (never, former). To maximize the relevance of the DNA adduct measurement as a proxy of PAH exposure, subjects selected had not changed residence, occupation, and major lifestyle factors during the last 10 years. Bulky DNA adducts were measured using the ³²P-postlabeling technique, nuclease P1 treatment. The percentage of detectable adducts was higher in controls (41%) than in cases (32%) with an odds ratio of 0.75 (95% confidence interval, 0.36-1.58). In an analysis limited to controls, a higher percentage of DNA adducts was found among those whose last residence was in a big city (50%) compared with those living in villages (19%; P = 0.04). No consistent associations were found for other markers of air pollution. In this study, among nonsmokers with stable environmental and lifestyle factors, bulky DNA adducts were not associated with bladder cancer risk. Results do not support an association of bladder cancer risk with low-level exposure to PAHs as measured through the formation of bulky DNA adducts in peripheral mononuclear cells. (Cancer Epidemiol Biomarkers Prev 2007;16(10):2155–9)