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Approaches to DNA/RNA Extraction and Whole Genome Amplification

Mailman School of Public Health, Columbia University, New York, New York

Requests for reprints: Regina M. Santella, Columbia University, 630 West 168th Street, New York, NY 10032. Phone: 212-305-1996; Fax: 212-305-5328. E-mail: rps1{at}columbia.edu

Analysis of DNA and/or RNA is an important component of most epidemiologic studies. The methods used for their preparation vary depending on the number of samples in the study as well as the amount of tissue or cells available, the specific downstream assay, and the resources available. They range from classic phenol/chloroform extractions to robotic methods suitable for large-scale studies. Several methods are also available for the quantitation of DNA/RNA, including UV or fluorescence measurements and PCR. For DNA samples of limited quantity, it is also now possible to use whole genome amplification to increase the amount of DNA suitable for genotyping. Finally, a major issue in quality control is the careful labeling and handling of samples. (Cancer Epidemiol Biomarkers Prev 2006;15(9):1585–7)

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