This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ahn, J. Articles by Ambrosone, C. B. Search for Related Content PubMed PubMed Citation Articles by Ahn, J. Articles by Ambrosone, C. B.

Associations between Catalase Phenotype and Genotype: Modification by Epidemiologic Factors

¹ Department of Epidemiology, Roswell Park Cancer Institute; ² Department of Biostatistics, State University of New York at Buffalo, Buffalo, New York; ³ University of Arkansas for Medical Sciences; ⁴ Central Arkansas Veterans Healthcare System, Little Rock, Arkansas; ⁵ Division of Pharmacogenomics and Molecular Epidemiology, National Center for Toxicological Research, Jefferson, Arkansas; and ⁶ Nutritional Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, NZH, Bethesda, Maryland

Requests for reprints: Christine Ambrosone, Department of Epidemiology, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263. Phone: 716-845-3082; Fax: 716-845-8125. E-mail: christine.ambrosone{at}roswellpark.org

Catalase is an endogenous antioxidant enzyme that neutralizes hydrogen peroxide and is induced by oxidative challenge. A –262C T polymorphism in the promoter region of the gene (CAT) is associated with risk of several conditions related to oxidative stress. We sought to determine the functional effects of the CAT polymorphism on enzyme activity in erythrocytes and the potential modifying effects of demographic and lifestyle factors on genotype/phenotype relationships, using specimens and data from controls from breast and prostate cancer studies in Arkansas (n = 420). There was a dose-response reduction in catalase activity by genotype, with geometric means of 115.4 units/mg hemoglobin for those with CC genotypes, 82.1 units/mg for those with CT genotypes, and 73.5 units/mg for those with TT genotypes. Associations were only observed among Caucasians (P < 0.0001), with no effects among African Americans (P = 0.91), and were stronger among women than men, although numbers in stratified analyses were small. Differences in catalase activity by genotype were most pronounced among those in the highest tertiles of consumption of fruits and vegetables (–35%, P = 0.003), with weaker relationships among those who were lower consumers (–21.8%, P = 0.16). Among those with CC genotypes, there was no change in activity by consumption, but there were notable decreases in activity by tertiles of consumption for those with at least one T allele. These data indicate that the CAT –262C T polymorphism predicts a portion of catalase phenotype, which may be limited to Caucasians. Associations between genotype and phenotype were modified by dietary factors, illustrating the biochemical complexity of studies of genetic polymorphisms and disease risk. (Cancer Epidemiol Biomarkers Prev 2006;15(6):1217-22)

This article has been cited by other articles:

				Y. Li, C. B. Ambrosone, M. J. McCullough, J. Ahn, V. L. Stevens, M. J. Thun, and C.-C. Hong Oxidative stress-related genotypes, fruit and vegetable consumption and breast cancer risk Carcinogenesis, May 1, 2009; 30(5): 777 - 784. [Abstract] [Full Text] [PDF]

				E. E Fabre, A. Raynaud-Simon, J.-L. Golmard, M. Hebert, X. Dulcire, M. Succari, J. Myara, D. Durand, and V. Nivet-Antoine Gene polymorphisms of oxidative stress enzymes: prediction of elderly renutrition Am. J. Clinical Nutrition, May 1, 2008; 87(5): 1504 - 1512. [Abstract] [Full Text] [PDF]

				S. K. Quick, P. G. Shields, J. Nie, M. E. Platek, S. E. McCann, A. D. Hutson, M. Trevisan, D. Vito, R. Modali, T. A. Lehman, et al. Effect Modification by Catalase Genotype Suggests a Role for Oxidative Stress in the Association of Hormone Replacement Therapy with Postmenopausal Breast Cancer Risk Cancer Epidemiol. Biomarkers Prev., May 1, 2008; 17(5): 1082 - 1087. [Abstract] [Full Text] [PDF]

				T. Islam, R. McConnell, W. J. Gauderman, E. Avol, J. M. Peters, and F. D. Gilliland Ozone, Oxidant Defense Genes, and Risk of Asthma during Adolescence Am. J. Respir. Crit. Care Med., February 15, 2008; 177(4): 388 - 395. [Abstract] [Full Text] [PDF]

				P. Rajaraman, A. Hutchinson, N. Rothman, P. M. Black, H. A. Fine, J. S. Loeffler, R. G. Selker, W. R. Shapiro, M. S. Linet, and P. D. Inskip Oxidative response gene polymorphisms and risk of adult brain tumors Neuro-oncol, January 1, 2008; 10(5): 709 - 715. [Abstract] [Full Text] [PDF]

				J.-Y. Choi, M. L. Neuhouser, M. Barnett, M. Hudson, A. R. Kristal, M. Thornquist, I. B. King, G. E. Goodman, and C. B. Ambrosone Polymorphisms in Oxidative Stress-Related Genes Are Not Associated with Prostate Cancer Risk in Heavy Smokers Cancer Epidemiol. Biomarkers Prev., June 1, 2007; 16(6): 1115 - 1120. [Abstract] [Full Text] [PDF]

				C. B. Ambrosone, P. G. Shields, J. L. Freudenheim, and C.-C. Hong Re: Commonly Studied Single-Nucleotide Polymorphisms and Breast Cancer: Results From the Breast Cancer Association Consortium J Natl Cancer Inst, March 21, 2007; 99(6): 487 - 487. [Full Text] [PDF]

				P. Pharoah Response: Re: Commonly Studied Single-Nucleotide Polymorphisms and Breast Cancer: Results From the Breast Cancer Association Consortium J Natl Cancer Inst, March 21, 2007; 99(6): 488 - 489. [Full Text] [PDF]

				J. Ahn, C. B. Ambrosone, P. A. Kanetsky, C. Tian, T. A. Lehman, S. Kropp, I. Helmbold, D. von Fournier, W. Haase, M. L. Sautter-Bihl, et al. Polymorphisms in Genes Related to Oxidative Stress (CAT, MnSOD, MPO, and eNOS) and Acute Toxicities from Radiation Therapy following Lumpectomy for Breast Cancer Clin. Cancer Res., December 1, 2006; 12(23): 7063 - 7070. [Abstract] [Full Text] [PDF]