This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hsieh, L.-L. Articles by Cheng, A.-J. Search for Related Content PubMed PubMed Citation Articles by Hsieh, L.-L. Articles by Cheng, A.-J.

The XRCC1 399Gln Polymorphism and the Frequency of p53 Mutations in Taiwanese Oral Squamous Cell Carcinomas¹

Departments of Public Health [L-L. H., H-T. C., P-F. W., M-C. C.] and Otolaryngology Oncology, Head and Neck Surgery [I-H. C., C-T. L.], Division of Hematology/Oncology Department of Internal Medicine [H-M. W.], and Department of Medical Technology [A-J. C.], Chang Gung University, Tao-Yuan 333, Taiwan; Departments of Pathology [S-M. J.], and Radiation Oncology [J. T-C. C.], Chang Gung Memorial Hospital, Tao-Yuan 333, Taiwan; and Taipei Chang Gung Memorial Hospital Head and Neck Oncology Group, Tao-Yuan, 333 Taiwan [L-L. H., I-H. C., C-T. L., H-M. W., S-M. J., J. T-C. C., M-C. C., A-J. C.].

DNA repair gene polymorphisms have been implicated as susceptibility factors in cancer development. It is possible that DNA repair polymorphisms may also influence the risk of gene mutation. The 399Gln polymorphism in the DNA repair gene XRCC1 has been indicated to have a contributive role in DNA adduct formation, sister chromatid exchange, and an increased risk of cancer development. Two hundred thirty-seven male oral squamous cell carcinomas (OSCCs) were included in a study to investigate the role of the XRCC1 194Trp, 280His, and 399Gln polymorphisms on p53 gene mutation. PCR-single-strand conformation polymorphism and DNA sequencing were used to analyze the conserved regions of the p53 gene (exons 5–9). The XRCC1 genotype was determined by PCR-RFLP. Nineteen (8.02%) of the 237 OSCCs had a Gln/Gln genotype. One hundred six (43.88%) of the 237 OSCCs showed p53 gene mutations at exons 5–9. The OSCC patients with a Gln/Gln genotype exhibited a significantly higher frequency of p53 mutation than those with an Arg/Gln and an Arg/Arg genotype. After adjustment for age, cigarette smoking, areca quid chewing, and alcohol drinking, the Gln/Gln genotype still showed an independent association with the frequency of p53 mutation (odd ratio, 4.50; 95% confidence interval, 1.52–13.36). The findings support the hypothesis that XRCC1 Arg399Gln amino acid change may alter the phenotype of the XRCC1 protein, resulting in a DNA repair deficiency. This study also suggests an important role for the XRCC1 399Gln polymorphism in p53 gene mutation in Taiwanese OSCCs.

This article has been cited by other articles:

				M. Majumder, N. Sikdar, R. R. Paul, and B. Roy Increased Risk of Oral Leukoplakia and Cancer Among Mixed Tobacco Users Carrying XRCC1 Variant Haplotypes and Cancer Among Smokers Carrying Two Risk Genotypes: One on Each of Two Loci, GSTM3 and XRCC1 (Codon 280) Cancer Epidemiol. Biomarkers Prev., September 1, 2005; 14(9): 2106 - 2112. [Abstract] [Full Text] [PDF]

				Z. Hu, H. Ma, F. Chen, Q. Wei, and H. Shen XRCC1 Polymorphisms and Cancer Risk: A Meta-analysis of 38 Case-Control Studies Cancer Epidemiol. Biomarkers Prev., July 1, 2005; 14(7): 1810 - 1818. [Abstract] [Full Text] [PDF]

				L.-E Wang, M. L. Bondy, H. Shen, R. El-Zein, K. Aldape, Y. Cao, V. Pudavalli, V. A. Levin, W. K. A. Yung, and Q. Wei Polymorphisms of DNA Repair Genes and Risk of Glioma Cancer Res., August 15, 2004; 64(16): 5560 - 5563. [Abstract] [Full Text] [PDF]

				J. C. Figueiredo, J. A. Knight, L. Briollais, I. L. Andrulis, and H. Ozcelik Polymorphisms XRCC1-R399Q and XRCC3-T241M and the Risk of Breast Cancer at the Ontario Site of the Breast Cancer Family Registry Cancer Epidemiol. Biomarkers Prev., April 1, 2004; 13(4): 583 - 591. [Abstract] [Full Text] [PDF]